Synthetic Gene Networks : Methods and Protocols / edited by Wilfried Weber, Martin Fussenegger.

Format:

Book

Contributor:

Weber, Wilfried, editor.

Fussenegger, Martin, editor.

SpringerLink (Online service)

Series:

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 813.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 813

Language:

English

Subjects (All):

Life sciences.

Bioinformatics.

Systems biology.

Life Sciences.

Systems Biology.

Local Subjects:

Life Sciences.

Bioinformatics.

Systems Biology.

Physical Description:

1 online resource (XI, 393 pages) : 67 illustrations, 3 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2012.

System Details:

text file PDF

Summary:

The rapid expansion of synthetic biology is due to the design and construction of synthetic gene networks that have opened many new avenues in fundamental and applied research. Synthetic Gene Networks: Methods and Protocols provides the necessary information to design and construct synthetic gene networks in different host backgrounds. Divided into four convenient sections, this volume focuses on design concepts to devise synthetic gene networks and how mathematical models can be applied to the predictable engineering of desired network features. The volume continues by highlighting the construction and validation of biologic tools, describing strategies to optimize and streamline the host cell for optimized network performance, and covering how optimally designed gene networks can be implemented in a large variety of host cells ranging from bacteria over yeast and insect cells to plant and mammalian cell culture. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Synthetic Gene Networks: Methods and Protocols serves as an invaluable resource for established biologists, engineers, and computer scientists or novices just entering into the rapidly growing field of synthetic biology.

Contents:

In silico Implementation of Synthetic Gene Networks

Standardization in Synthetic Biology

Robust Optimal Design of Synthetic Biological Networks

Predicting Synthetic Gene Networks

Reprogramming a GFP Reporter Gene Subjects it to Complex Lentiviral Gene Regulation

A High-throughput Microfluidic Method for Generating and Characterizing Transcription Factor Mutant Libraries

Identifying and Optimizing Intracellular Protein-protein Interactions using Bacterial Genetic Selection

Zinc-finger Nucleases Based Genome Engineering to Generate Isogenic Human Cell Lines

RNA-based Networks: Using RNA Aptamers and Ribozymes as Synthetic Genetic Devices

MicroRNA Circuits for Transcriptional Logic

Light-regulated Gene Expression in Yeast

Light-controlled Gene Switches in Mammalian Cells

Expressed Protein Modifications: Making Synthetic Proteins

Using Transcription Machinery Engineering to Elicit Complex Cellular Phenotypes

Streamlining of a Pseudomonas putida Genome using a Combinatorial Deletion Method Based on Mini-transposon Insertion and the Flp-FRT Recombination System

Transposon-based and Plasmid-based Genetic Tools for Editing Genomes of Gram-negative Bacteria

Synthetic Networks: Oscillators and Toggle Switches for Escherichia coli

Studying Microbial Communities in Biofilms

Quantitative Analysis of the Spatiotemporal Dynamics of a Synthetic Predator-prey Ecosystem

Drosophila S2 Schneider Cells - A Useful Tool for Rebuilding and Redesigning Approaches in Synthetic Biology

Synthetic Gene Networks in Plant Systems

Design and Construction of Synthetic Gene Networks in Mammalian Cells

Synthetic Gene Networks as Blueprint for Smart Hydrogels.

Other Format:

Printed edition:

ISBN:

9781617794124

Access Restriction:

Restricted for use by site license.