Arthritis Research : Methods and Protocols Volume 2 / edited by Andrew P. Cope.

Format:

Book

Contributor:

Cope, Andrew P., editor.

SpringerLink (Online service)

Series:

Methods in molecular medicine 1543-1894 ; 136.

Springer Protocols (Springer-12345)

Methods in Molecular Medicine, 1543-1894 ; 136

Language:

English

Subjects (All):

Medicine.

Human genetics.

Orthopedics.

Cytology.

Medicine & Public Health.

Human Genetics.

Cell Biology.

Local Subjects:

Medicine & Public Health.

Orthopedics.

Human Genetics.

Cell Biology.

Physical Description:

1 online resource (XXII, 432 pages) : 79 illustrations, 6 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2007.

System Details:

text file PDF

Summary:

Arthritis Research: Methods and Protocols is a compendium of data pertinent to the methods and protocols that have contributed to recent advances in molecular medicine in general, but to the molecular basis of rheumatic disease in particular. These volumes details novel technologies, some of which are still evolving and whose impacts are yet to be determined. Leaders in the field contribute to cover such exciting and cutting edge topics as imaging and immunohistochemistry, analysis of cartilage and bone catabolism, immunobiology, and cell trafficking. In Volume 1, authors discuss synovial joint morphology, histopathology, and immunohistochemistry, cartilage matrix and bone biology, and cell trafficking, migration and invasion. Volume 2 is broken up into sections that cover immunobiology and T cells, animal models of arthritis, and applications of new technologies, such as Differential Display Reverse Transcription-Polymerase Chain Reaction (DDRT-PCR), to define novel therapeutic targets. Both volumes combine to produce a concise set of protocols condensing decades of experience and expertise. Arthritis Research: Methods and Protocols will be a valuable tool for basic research investigators, clinician scientists, pathologists, immunologists, and biochemists looking to stay current in their fields.

Contents:

Immunobiology

Phenotypic Analysis of B-Cells and Plasma Cells

Detection of Antigen Specific B-Cells in Tissues

Single Cell Analysis of Synovial Tissue B-Cells

Tracking Antigen Specific CD4+ T-Cells With Soluble MHC Molecules

Analysis of Antigen Reactive T-Cells

Identification and Manipulation of Antigen Specific T-Cells with Artificial Antigen Presenting Cells

Analysis of Th1/Th2 T-Cell Subsets

Analysis of the T-Cell Receptor Repertoire of Synovial T-Cells

The Assessment of T-Cell Apoptosis in Synovial Fluid

Assays of T-Cell Contact Dependent Monocyte-Macrophage Functions

Phenotypic and Functional Analysis of Synovial Natural Killer Cells

Identification and Isolation of Synovial Dendritic Cells

Animal Models of Arthritis

The Use of Animal Models for Rheumatoid Arthritis

Collagen-Induced Arthritis in Mice

Collagen-Induced Arthristis in Rats

Collagen Antibody Induced Arthritis

Arthritis Induced with Minor Cartilage Proteins

Murine Antigen-Induced Arthritis

Pristane-Induced Arthritis in the Rat

The K/BxN Mouse Model of Inflammatory Arthritis

Analysis of Arthritic Lesions in the Del1 Mouse

Application of New Technologies to Define Novel Therapeutic Targets

Gene Expression Profiling in Rheumatology

Differential Display Reverse Transcription-Polymerase Chain Reaction to Identify Novel Biomolecules in Arthritis Research

Two-Dimensional Electrophoresis of Proteins Secreted from Articular Cartilage

Mapping Lymphocyte Plasma Membrane Proteins

In Vivo Phage Display Selection in the Human/SCID Mouse Chimera Model for Defining Synovial Specific Determinants

Adenoviral Targeting of Signal Transduction Pathways in Synovial Cell Cultures.

Other Format:

Printed edition:

ISBN:

9781597454025

Access Restriction:

Restricted for use by site license.