Hypertension : Methods and Protocols / edited by Jérôme P. Fennell, Andrew H. Baker.

Format:

Book

Contributor:

Fennell, Jérôme P., editor.

Baker, Andrew H., editor.

SpringerLink (Online service)

Series:

Methods in molecular medicine 1543-1894 ; 108.

Springer Protocols (Springer-12345)

Methods In Molecular Medicine™, 1543-1894 ; 108

Language:

English

Subjects (All):

Medicine.

Cardiology.

Medicine & Public Health.

Local Subjects:

Medicine & Public Health.

Cardiology.

Physical Description:

1 online resource (XVI, 502 pages) : 98 illustrations.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2005.

System Details:

text file PDF

Summary:

Our understanding of the pathogenesis of hypertension and the development of new therapies for its management have each proven to be directly dependent on the availability and application of novel molecular biological methodologies. In Hypertension: Methods and Protocols, a panel of recognized experts from key institutions around the world describes in step-by-step detail many new and essential molecular techniques for cardiovascular hypertension research. The methods presented range widely from producing congenic, consomic, transgenic, and knockout models of hypertension to the gene transfer of specific genetic material using adenoviral and nonviral (polymers, liposomes, and antisense agents) vectors. Additional techniques described include single nucleotide polymorphism (SNP) genotyping, RNA interference, microarray analysis, pharmacogenetics, and pharmacogenomics for the genetic dissection of hypertension, as well as a practical method for deriving cardiomyocytes from embryonic stem cells that would serve as replacement cells for those damaged by hypertension or heart attack. A review of bioinformatic resources for pharmacogenomics describes the application of in silico strategies to identify nuclear matrix attachment regions. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Hypertension: Methods and Protocols offers both novice and experienced hypertension researchers an indispensable collection of readily reproducible techniques for successful research, work that has already dramatically improved the outlook for hypertensive patients, and promises much future success.

Contents:

Models Of Hypertension

Congenic/Consomic Models of Hypertension

Mouse Knockout Models of Hypertension

Production of Transgenic Models in Hypertension

Measuring Blood Pressure in Small Laboratory Animals

Assessment Of Free Radicals In Endothelial Dysfunction

Analysis of Superoxide Anion Production in Tissue

Measurement of Vascular Reactive Oxygen Species Production by Chemiluminescence

A Guide to Wire Myography

Nucleic Acid Techniques

Selection of Candidate Genes in Hypertension

Genome-Wide Scanning With SSLPs in the Rat

Extraction of RNA From Cells and Tissue

Single-Strand Conformational Polymorphism Analysis

Single-Nucleotide Polymorphism Genotyping for Disease Association Studies

Denaturing High-Performance Liquid Chromatography Using the WAVE DNA Fragment Analysis System

TaqMan Real-Time PCR Quantification Conventional and Modified Methods

Generation of High-Sensitivity Antisense cDNA Probes by Asymmetric PCR

New Advances in Microarrays Finding the Genes Causally Involved in Disease

Pharmacogenetics and Pharmacogenomics

Proteins And Proteomics

Determination of the Endogenous Nitric Oxide Synthase Inhibitor Asymmetric Dimethylarginine in Biological Samples by HPLC

Proteomic Approaches in the Analysis of Hypertension

Gene Transfer

Gene Transfer in Endothelial Dysfunction and Hypertension

Nonviral Gene Delivery Methods in Cardiovascular Diseases

Construction and Characterization of Helper-Dependent Adenoviral Vectors for Sustained In Vivo Gene Therapy

Adenovirus-Mediated Gene Transfer In Vivo An Approach to Reduce Oxidative Stress

Gene Therapy for Hypertension

Modulation of Gene Expression by RNAi

In Vivo Biopanning

Stem Cells

Cardiomyocytes Derived From Embryonic Stem Cells

Bioinformatics

In Silico and Wet-Bench Identification of Nuclear Matrix Attachment Regions

Biomedical Informatics Methods in Pharmacogenomics.

Other Format:

Printed edition:

ISBN:

9781592598502

Access Restriction:

Restricted for use by site license.