Wound Healing : Methods and Protocols / edited by Luisa A. DiPietro, Aime L. Burns.

Format:

Book

Contributor:

DiPietro, Luisa A., editor.

Burns, Aime L., editor.

SpringerLink (Online service)

Series:

Methods in molecular medicine 1543-1894 ; 78.

Springer Protocols (Springer-12345)

Methods in Molecular Medicine™, 1543-1894 ; 78

Language:

English

Subjects (All):

Medicine.

Surgery.

Medicine & Public Health.

Local Subjects:

Medicine & Public Health.

Surgery.

Physical Description:

1 online resource (XVI, 468 pages).

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press : Imprint: Humana Press, 2003.

System Details:

text file PDF

Summary:

During the past decade the significant progress made in cell and molecular biotechnology has led to exciting discoveries in the field of tissue repair, producing a wealth of information about the healing process. In Wound Healing: Methods and Protocols, expert researchers and physicians not only describe classic and contemporary laboratory methods for studying tissue repair using a broad range of wound healing models, but also provide sensitive assays for the assessment of tissue healing. The systems detailed include human, animal, in vitro, in vivo, and impaired healing models, as well as models in which underlying systemic and genetic conditions influence the healing process. Useful for the study of many biological processes, including angiogenesis, epidermal differentiation and repair, acute inflammation, ECM synthesis, and remodeling, these powerful models illustrate biochemical, molecular, and surgical techniques designed for the analysis and manipulation of the healing wound, and provide step-by-step instructions to ensure successful results. In many cases, several different approaches to a single process are examined, with sample results and analyses given to help users in selecting the approach most suited to the problem at hand. Comprehensive and enlightening, Wound Healing: Methods and Protocols offers both basic and clinical scientists a thought-provoking and highly practical collection of widely used model systems and methods for studying the injury repair process.

Contents:

Experimental Models of Wound Healing

Excisional Wound Healing

Methods in Reepithelialization

Incisional Wound Healing

Animal Models of Ischemic Wound Healing

Corneal Injury

Subcutaneous Sponge Models

A Mouse Model of Burn Wounding and Sepsis

A Porcine Burn Model

Wound Healing in Airways In Vivo

Murine Models of Intestinal Anastomoses

Murine Model of Peritoneal Adhesion Formation

Methods for Investigating Fetal Tissue Repair

Growth of Human Blood Vessels in Severe Combined Immunodeficient Mice

Tissue Repair in Models of Diabetes Mellitus

Wound Healing Studies in Transgenic and Knockout Mice

Wound Repair in Aging

Specimen Collection and Analysis

Suction Blister Model of Wound Healing

Implantable Wound Healing Models and the Determination of Subcutaneous Collagen Deposition in Expanded Polytetrafluoroethylene Implants

The Fibroblast-Populated Collagen Lattice

A Quantifiable In Vitro Model to Assess Effects of PAI-1 Gene Targeting on Epithelial Cell Motility

Human Skin Organ Culture

In Vitro Matrigel Angiogenesis Model

Analysis and Manipulation of Wound Healing

Quantification of Wound Angiogenesis

In Vivo Matrigel Migration and Angiogenesis Assays

Endothelial Cell Migration Assay

Analysis of Collagen Synthesis

Method for Detection and Quantitation of Leukocytes During Wound Healing

Detection of Reactive Oxygen Intermediate Production by Macrophages

Measurement of Chemokines at the Protein Level in Tissue

Methods of Measuring Oxygen in Wounds

Isolation, Culture, and Characterization of Human Intestinal Smooth Muscle Cells

Use of High-Throughput Microarray Membranes for cDNA Analysis of Cutaneous Wound Repair

Particle-Mediated Gene Therapy of Wounds.

Other Format:

Printed edition:

ISBN:

9781592593323

Access Restriction:

Restricted for use by site license.