Candida albicans : Methods and Protocols / edited by Ronald L. Cihlar, Richard A. Calderone.

Format:

Book

Contributor:

Cihlar, Ronald L., editor.

Calderone, Richard A., 1942- editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 499.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, 1064-3745 ; 499

Language:

English

Subjects (All):

Life sciences.

Proteomics.

Microbiology.

Bacteriology.

Life Sciences.

Local Subjects:

Life Sciences.

Microbiology.

Bacteriology.

Proteomics.

Physical Description:

1 online resource (X, 206 pages) : 28 illustrations.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press : Imprint: Humana Press, 2009.

System Details:

text file PDF

Summary:

Over the course of the past decade, there have been remarkable advances in the study of human pathogenic fungi. These developments have taken place throughout a wide range of disciplines, and have come as the result of newly available genome sequences of pathogens such as candida albicans and other model fungi. In Candida Albicans: Methods and Protocols, expert researchers explore these exciting new insights, focusing on the study of medically important fungi and Candida spp in particular. Chapters examine critical aspects of molecular methods, providing information on reporter gene assays, transformation, gene expression in vivo, and methods for large-scale gene disruption. At the same time, the work includes in-depth descriptions of disease models of candidiaisis, facts about strain identification, and guidelines on the preparation of samples for proteomic investigations and tandem affinity purification. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting edge, Candida Albicans: Methods and Protocols is an invaluable source of methods for investigators in the exhilarating fields of medical and molecular mycology.

Contents:

Immunogical Methods

Isolation of Dendritic Cells from Human Blood for In Vitro Interaction Studies with Fungal Antigens

Detection and Quantitation of Antifungal SIgA Antibodies in Body Fluids

Phagocytosis and Killing Assays for Candida Species

Immunization Protocols for Use in Animal Models of Candidiasis

Virulence and Biofilms

Penetration of Antifungal Agents Through Candida Biofilms

Candida Biofilm Analysis in the Artificial Throat Using FISH

Conditions for Optimal Candida Biofilm Development in Microtiter Plates

Virulence Measurements: In vitro, ex vivo, and in vivo

Animal Models of Candidiasis

Candida albicans Gene Expression in an In Vivo Infection Model

In Vitro and Ex Vivo Assays of Virulence in Candida albicans

Strain Typing and Identification

Biotyping of Candida albicans and Other Fungi by Yeast Killer Toxins Sensitivity

DNA Fingerprinting Candida Species

Genomics and Proteomics

The Application of Tandem-Affinity Purification to Candida albicans

Preparation of Samples for Proteomic Analysis of the Candida albicans Cell Wall

Reporter Gene Assays in Candida albicans

Genetic Transformation of Candida albicans

Large-Scale Gene Disruption Using the UAU1 Cassette

Standard Growth Media and Common Techniques for Use with Candida albicans.

Other Format:

Printed edition:

ISBN:

9781603271516

Access Restriction:

Restricted for use by site license.