Dendritic Cell Protocols / edited by Stephen P. Robinson, Andrew J. Stagg, Stella C. Knight.

Format:

Book

Contributor:

Robinson, S. P. (Stephen P.), editor.

Stagg, Andrew J., editor.

Knight, Stella C., editor.

SpringerLink (Online service)

Series:

Methods in molecular medicine 1543-1894 ; 64.

Springer Protocols (Springer-12345)

Methods in Molecular Medicine™, 1543-1894 ; 64

Language:

English

Subjects (All):

Medicine.

Immunology.

Biomedicine.

Local Subjects:

Biomedicine.

Immunology.

Physical Description:

1 online resource (XVII, 470 pages).

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2001.

System Details:

text file PDF

Summary:

It is now apparent that dendritic cells not only play important roles in the body's immune system through their complex interactions with T cells, B cells, and other cell types, but also possess distinct functional attributes that enable them to assume different roles in that system. In Dendritic Cell Protocols, Stephen P. Robinson, MD, PhD, and Andrew Stagg, PhD, have brought together a wide range of time-proven methods for studying these so-called "veiled" cells. Many of these readily reproducible techniques deal with the problem of obtaining sufficient dendritic cells for analysis, whether by isolation from a wide variety of tissues or from various progenitor cell populations. Other methods describe in step-by-step fashion the techniques commonly used for analyzing aspects of dendritic cells, ranging from cell migration to antigen uptake and T cell stimulation. Variant methods that have been successful in other laboratories have been included to expand experimental possibilities. In addition, a few techniques explore the practical challenges involved in using dendritic cells in a clinical setting to develop novel immunotherapeutics. State-of-the-art and highly practical, Dendritic Cell Protocols provides both experienced and novice investigators with powerful tools to illuminate the complex biology of these foundation cells-cells that shape the evolution of acquired immune responses, play important roles in innate immunity, and promise the development of powerful new immunotherapeutics.

Contents:

Isolation of Dendritic Cells from Animal Tissues

Isolation of Dendritic Cells from Mouse Lymph Nodes

Isolation of Mouse Spleen Dendritic Cells

Isolation of Mouse Thymic Dendritic Cells

Isolation of Dendritic Cells from Rat Intestinal Lymph and Spleen

Isolation, Enrichment, and Culture of Murine Epidermal Langerhans Cells

Murine Intestinal Dendritic Cell Isolation

Identification and Isolation of Rodent Lung Dendritic Cells

Isolation and Propagation of Mouse Liver-Derived Dendritic Cells

Isolation and Characterization of Human Dendritic Cells

Identification and Immunophenotypic Analyses of Peripheral Blood Dendritic Cells

Purification of Dendritic Cells from Peripheral Blood

Isolation of Human Tonsillar Dendritic Cells

Isolation of Dendritic Cells from Human Afferent Lymph

Isolation of Human Skin Dendritic Cells by In Vitro Migration

Human Intestinal Dendritic Cell Isolation

Isolation of Human Lung Dendritic Cells

Identification and Isolation of Synovial Dendritic Cells

Derivation of Animal Dendritic Cells from Progenitor Cells

Generation of Murine Bone-Marrow-Derived Dendritic Cells

Generation of Dendritic Cells from Rat Bone Marrow

Derivation of Murine Dendritic Cells from Thymic Precursors

Generation of Murine Dendritic Cell Lines

Generation of Mouse Dendritic Cell Lines

Generation of Langerhans Cell-Like Dendritic Cells from Murine Fetal Skin

Derivation of Dendritic Cell Lines from Mouse Skin

Generation of Human Dendritic Cells from Progenitor Cells

Propagation of Human Dendritic Cells In Vitro

Generation of Dendritic Cells from Lymphoid Precursors

Dendritic Cell Generation from Highly Purified CD14+Monocytes

Generation of Blood-Derived Human Dendritic Cells for Antitumor Immunotherapy

Aspects of Dendritic Cell Function

Chemotaxis of In Vitro Cultured Human Dendritic Cells

Adhesion of Dendritic Cells to Endothelia

Transendothelial Migration and Reverse Transmigration of In Vitro Cultured Human Dendritic Cells

In Vivo Assays of Langerhans Cell Migration

In Vivo Migration of Rat Dendritic Cells

Antigen Uptake by Dendritic Cells

Uptake of Antigen-Antibody Complexes by Human Dendritic Cells

Immunoelectron Microscopy of Antigen Processing in Dendritic Cells

Purification and Characterization of MHC Class II Containing Organelles in Mouse Bone-Marrow-Derived Dendritic Cells

Detection of Intracellular Cytokines in Dendritic Cells

Adenoviral-Vector-Mediated Gene Transfer to Dendritic Cells

Stimulation of Anti-HIV-1 Cytotoxic T Lymphocytes by Dendritic Cells.

Other Format:

Printed edition:

ISBN:

9781592591503

Access Restriction:

Restricted for use by site license.