RNA Detection and Visualization : Methods and Protocols / edited by Jeffrey E. Gerst.

Format:

Book

Contributor:

Gerst, Jeffrey E., editor.

SpringerLink (Online service)

Series:

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 714.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 714

Language:

English

Subjects (All):

Life sciences.

Nucleic acids.

Cytology.

Life Sciences.

Cell Biology.

Nucleic Acid Chemistry.

Local Subjects:

Life Sciences.

Cell Biology.

Nucleic Acid Chemistry.

Physical Description:

1 online resource (XIV, 498 pages).

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press : Imprint: Humana Press, 2011.

System Details:

text file PDF

Summary:

With its complex and extensively regulated metabolism, the study of the RNA lifecycle demands tools that allow for the localization of RNAs to be observed either in an in situ setting or, preferably, under in vivo conditions. In RNA Detection and Visualization: Methods and Protocols, the best and brightest investigators provide an up-to-date and in-depth description of basic methods and protocols used for detecting and visualizing mRNAs in both fixed and live cells, from bacteria to mammals. For novices and experts alike, this mix of classic in situ hybridization and advanced live imaging techniques, cell fractionation and affinity purification procedures, and bioinformatics tools gives researchers the most complete and extensive array of research aids possible. As a volume written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNA Detection and Visualization: Methods and Protocols offers well-honed techniques in order to inspire researchers around the world to further our knowledge of the vital biological significance of RNA.

Contents:

Single Molecule Imaging of RNA In situ

FISH and Immunofluorescence Staining in Chlamydomonas

High Resolution Fluorescent In situ Hybridization in Drosophila

Localization and Anchorage of Maternal mRNAs to Cortical Structures of Ascidian Eggs and Embryos Using High Resolution In situ Hybridization

Visualization of mRNA Localization in Xenopus Oocytes

Visualization of mRNA Expression in the Zebrafish Embryo

High-Resolution Fluorescence In situ Hybridization to Detect mRNAs in Neuronal Compartments In vitro and In vivo

Localization of mRNA in Vertebrate Axons Using In situ Hybridization

Tiny Molecular Beacons for In vivo mRNA Detection

Delivery of Molecular Beacons for Live-Cell Imaging and Analysis of RNA

Genetically-Encoded Fluorescent Probes for Imaging Endogenous mRNA in Living Cells

Visualization of Induced RNA in Single Bacterial Cells

Visualizing mRNAs in Fixed and Living Yeast Cells

In vivo Visualization of RNA Using the U1A-Based Tagged RNA System

Visualizing Endogenous mRNAs in Living Yeast Using m-TAG, a PCR-Based RNA Aptamer Integration Method, and Fluorescence Microscopy

Imaging mRNAs in Living Mammalian Cells

Using the mRNA-MS2/MS2CP-FP System to Study mRNA Transport during Drosophila Oogenesis

Genome-Wide Analysis of RNA Extracted from Isolated Mitochondria

Analyzing mRNA Localization to the Endoplasmic Reticulum via Cell Fractionation

Isolation of mRNAs Encoding Peroxisomal Proteins from Yeast Using a Combined Cell Fractionation and Affinity Purification Procedure

Profiling Axonal mRNA Transport

RNA Purification from Tumor Cell Protrusions Using Porous Polycarbonate Filters

RNA-Binding Protein Immunopurification-Microarray (RIP-Chip) Analysis to Profile Localized RNAs

RaPID: An Aptamer-Based mRNA Affinity Purification Technique for the Identification of RNA and Protein Factors Present in Ribonucleoprotein Complexes

RIP: An mRNA Localization Technique

The Dual Use of RNA Aptamer Sequences for Affinity Purification and Localization Studies of RNAs and RNA-Protein Complexes

Identifying and Searching for Conserved RNA Localization Signals

Computational Prediction of RNA Structural Motifs Involved in Post Transcriptional Regulatory Processes.

Other Format:

Printed edition:

ISBN:

9781617790058

Access Restriction:

Restricted for use by site license.