Spermatogenesis : Methods and Protocols / edited by Douglas T. Carrell, Kenneth I. Aston.

Format:

Book

Contributor:

Carrell, Douglas T., editor.

Aston, Kenneth I., editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 927.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, 1064-3745 ; 927

Language:

English

Subjects (All):

Cytology.

Embryology.

Developmental biology.

Cell Biology.

Developmental Biology.

Local Subjects:

Cell Biology.

Embryology.

Developmental Biology.

Physical Description:

1 online resource (XVII, 554 pages) : 110 illustrations, 68 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press : Imprint: Humana Press, 2013.

System Details:

text file PDF

Summary:

Deficiencies in sperm function are usually the result of spermatogenic defects. Spermatogenesis is a biologically complex and essential process during which spermatogonia undergo meiotic recombination, reduction of the genome to a haploid state, and extensive cellular modifications that result in a motile cell capable of traversing the female reproductive tract, withstanding various potential assaults to viability, and finally successfully fertilizing a mature oocyte to give rise to an embryo. Defects in any step of spermatogenesis or spermatogenesis can lead to male infertility, a disease that affects approximately 5-7% of the population. Spermiogenesis and Spermatogenesis: Methods and Protocols details protocols used in the study of spermatogenesis, clinical analytical protocols, and basic techniques used in clinical andrology laboratories, such as obtaining accurate results for a sperm count, and advanced procedures, such as genome-wide genetic study tools and evaluation of nuclear proteins. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Spermiogenesis and Spermatogenesis: Methods and Protocols is unique in its breadth, and will be a useful reference for clinicians and researchers alike.

Contents:

Methods For Sperm Concentration Determination

Methods of Sperm Vitality Assessment

The Hypo-osmotic Swelling Test for Evaluation of Sperm Membrane Integrity

Sperm Morphology Classification: A Rational Method For Schemes Adopted By The World Health Organization

Sperm Morphology Assessment Using Strict (Tygerberg) Criteria

Methods For Direct And Indirect Antisperm Antibody Testing

Manual Methods For Sperm Motility Assessment

Computer-Aided Sperm Analysis (CASA) Of Sperm Motility And Hyperactivation

The Hemizona Assay For Assessment Of Sperm Function

The Sperm Penetration Assay for the Assessment of Fertilization Capacity

Methods For The Assessment Of Sperm Capacitation And Acrosome Reaction Excluding The Sperm Penetration Assay

Sperm DNA Fragmentation Analysis Using the TUNEL Assay

Sperm DNA Damage Measured by Comet Assay

Sperm Chromatin Structure Assay (SCSA®)

Sperm Aneuploidy Testing Using Fluorescence In Situ Hybridization (FISH)

Flow Cytometric Methods For Sperm Assessment

Human Y Chromosome Microdeletion Analysis By PCR Multiplex Protocols Identifying Only Clinically Relevant AZF Microdeletions

Sperm Cryopreservation Methods

Density Gradient Separation Of Sperm For Artificial Insemination

Recovery, Isolation, Identification and Preparation of Spermatozoa from Human Testis

Enhancement of Sperm Motility Using Pentoxifylline and Platelet-Activating Factor

Intracytoplasmic Morphology Selected Sperm Injection

Sperm Selection For ICSI Using Annexin V

Sperm Selection for ICSI Using the Hyaluronic Acid Binding Assay

Sperm Selection Based On Electrostatic Charge

Sex-Sorting Sperm Using Flow Cytometry/Cell Sorting

Assessment of Spermatogenesis Through Staging of Seminiferous Tubules

Immunohistochemical Approaches for the Study of Spermatogenesis

Ultrastructural Analysis of Testicular Tissue and Semen by Transmission and Scanning Electron Microscopy

Assessment Of Oxidative Stress In Sperm And Semen

Improved Chemiluminescence Assay for Measuring Antioxidant Capacity of Seminal Plasma

Methods of Sperm DNA Extraction for Genetic and Epigenetic Studies

Isolating mRNA and Small Noncoding RNAs From Human Sperm

A Review Of Genome-Wide Approaches To Study The Genetic Basis For Spermatogenic Defects

Methods For The Analysis Of The Sperm Proteome

Methodology of Aniline Blue Staining Of Chromatin and the Assessment of the Associated Nuclear and Cytoplasmic Attributes in Human Sperm

Isolation of Sperm Nuclei and Nuclear Matrices from the Mouse, and Other Rodents

Extraction and Analysis of Human Sperm Protamine 1 / Protamine 2 Ratio Using Acid Gel Electrophoresis

Analysis of Gene-Specific and Genome-Wide Sperm DNA Methylation

Evaluating the Localization and DNA Binding Complexity of Histones in Mature Sperm

In Vitro Spermatogenesis Using An Organ Culture Technique

Testicular Tissue Grafting and Male Germ Cell Transplantation

Transgenic Modification of Spermatogonial Stem Cells Using Lentiviral Vectors

Methods for Sperm-Mediated Gene Transfer

Phenotypic Assessment of Male Fertility Status in Transgenic Animal Models.

Other Format:

Printed edition:

ISBN:

9781627030380

Access Restriction:

Restricted for use by site license.