2 options
Drosophila : Methods and Protocols / edited by Christian Dahmann.
Holman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
Available
- Format:
- Book
- Series:
- Methods in molecular biology 1064-3745 ; 420.
- Springer Protocols (Springer-12345)
- Methods in Molecular Biology, 1064-3745 ; 420
- Language:
- English
- Subjects (All):
- Life sciences.
- Cytology.
- Animal genetics.
- Entomology.
- Life Sciences.
- Cell Biology.
- Animal Genetics and Genomics.
- Local Subjects:
- Life Sciences.
- Entomology.
- Cell Biology.
- Animal Genetics and Genomics.
- Physical Description:
- 1 online resource (XII, 438 pages) : 101 illustrations, 13 illustrations in color.
- Contained In:
- Springer eBooks
- Place of Publication:
- Totowa, NJ : Humana Press, 2008.
- System Details:
- text file PDF
- Summary:
- With its long-standing tradition as a model organism, many techniques using Drosophila melanogaster have been established and continue to be developed, while the recent invention of techniques allowing the knock-down of genes by RNA interference and gene replacement by homologous recombination demonstrates the ongoing efforts to broaden the technical repertoire. In Drosophila: Methods and Protocols, leading experts contribute a collection of protocols covering standard techniques as well as novel methods. As a volume in the highly successful Methods in Molecular Biology™ series, the chapters contain brief introductions to the subject matter, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and essential, Drosophila: Methods and Protocols allows novices and experts alike to tap into the power of one hundred years of expertise involving this vital model system.
- Contents:
- Drosophila melanogaster and the Development of Biology in the 20th Century
- Getting Started
- FlyBase
- The Use of P-Element Transposons to Generate Transgenic Flies
- The GAL4 System
- P-Element Mutagenesis
- EMS Screens
- RNAi Screening in Cultured Drosophila Cells
- Methods for Homologous Recombination in Drosophila
- Recombinases and Their Use in Gene Activation, Gene Inactivation, and Transgenesis
- Cuticle Preparation of Drosophila Embryos and Larvae
- Immunolabeling of Embryos
- Imaging Cellular and Molecular Dynamics in Live Embryos Using Fluorescent Proteins
- Analysis of Neuromuscular Junctions
- Immunolabeling of Imaginal Discs
- Imaging Drosophila Pupal Wing Morphogenesis
- Microscopic Analysis of the Adult Drosophila Retina Using Semithin Plastic Sections
- Fluorescent In Situ Hybridization Protocols in Drosophila Embryos and Tissues
- Linear RNA Amplification for the Production of Microarray Hybridization Probes
- An Overview of the Identification, Detection, and Functional Analysis of Drosophila MicroRNAs
- Extraction and Immunoblotting of Proteins From Embryos
- Purification of Drosophila Protein Complexes for Mass Spectrometry
- Mass Production of Drosophila Embryos and Chromatographic Purification of Native Protein Complexes
- Flow Cytometric Analysis of Drosophila Cells
- Drosophila Cell Lines as Model Systems and as an Experimental Tool.
- Other Format:
- Printed edition:
- ISBN:
- 9781597455831
- Access Restriction:
- Restricted for use by site license.
The Penn Libraries is committed to describing library materials using current, accurate, and responsible language. If you discover outdated or inaccurate language, please fill out this feedback form to report it and suggest alternative language.