2 options
PRINS and In Situ PCR Protocols / edited by John R. Gosden.
Holman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
Available
- Format:
- Book
- Series:
- Methods in molecular biology 1064-3745 ; 71.
- Springer Protocols (Springer-12345)
- Methods in Molecular Biology™, 1064-3745 ; 71
- Language:
- English
- Subjects (All):
- Life sciences.
- Cytology.
- Life Sciences.
- Cell Biology.
- Local Subjects:
- Life Sciences.
- Cell Biology.
- Physical Description:
- 1 online resource (XIII, 165 pages) : 75 illustrations, 22 illustrations in color.
- Contained In:
- Springer eBooks
- Place of Publication:
- Totowa, NJ : Humana Press, 1997.
- System Details:
- text file PDF
- Summary:
- Cutting edge researchers demonstrate step-by-step how oligonucleotide primers may be successfully used to detect and amplify or extend complimentary sequences in situ. Through these procedures-often invented by the authors-the door is opened to rapid identification and characterization of chromosomal DNA sequences, viral genomes, and rare messenger RNAs in cells-at hitherto unmatched degrees of sensitivity and specificity. Their innovative techniques-suitable for both novice and experienced researchers-have rapidly become indispensable for many clinical diagnostic procedures, whether in quantification of chromosomes in the identification of aneuploidy for prenatal diagnosis, or in the identification of viral infection in the early stages, or of rare messenger RNAs present in cells.
- Contents:
- Oligonucleotide PRINS DNA Synthesis
- Chromosome-Specific PRINS
- Bright-Field Microscopic Detection of Oligonucleotide PRINS-Labeled DNA in Chromosome Preparations
- Analysis of Sperm Aneuploidy by PRINS
- PRINS DNA Synthesis on Frozen Tissue Sections
- Multiple Sequential Oligonucleotide Primed In Situ DNA Syntheses (MULTI-PRINS)
- Oligonucleotide PRINS DNA Synthesis on Extended Chromatin Preparations
- Combined lmmunocytochemistry and PRINS DNA Synthesis for Simultaneous Detection of Phenotypic and Genomic Parameters in Cells
- Chromosomal PRINS DNA Labeling Combined with Indirect Immunocytochemistry
- Direct In Situ Single-Copy (DISC)-PCR
- In Situ PCR to Cells and to Wax Sections
- Reverse Transcriptase PCR In Situ on Cryopreserved Tissue Sections
- Flow Cytometry Analysis of an In Situ PCR for the Detection of Human Immunodeficiency Virus Type-1 (HIV-1) Proviral DNA
- In Situ PCR Amplification of Intracellular mRNA
- Localized In Situ Amplification (LISA)
- In Situ PCR.
- Other Format:
- Printed edition:
- ISBN:
- 9781592595570
- Access Restriction:
- Restricted for use by site license.
The Penn Libraries is committed to describing library materials using current, accurate, and responsible language. If you discover outdated or inaccurate language, please fill out this feedback form to report it and suggest alternative language.