Protein-Protein Interactions : Methods and Applications / edited by Cheryl L. Meyerkord, Haian Fu.

Format:

Book

Contributor:

Meyerkord, Cheryl L., editor.

Fu, Haian, editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1278.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, 1064-3745 ; 1278

Language:

English

Subjects (All):

Life sciences.

Proteins.

Life Sciences.

Protein Science.

Local Subjects:

Life Sciences.

Protein Science.

Physical Description:

1 online resource (XV, 620 pages) : 139 illustrations, 70 illustrations in color.

Edition:

Second edition 2015.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2015.

System Details:

text file PDF

Summary:

In recent years, our understanding of the protein-protein interaction landscape has expanded significantly. Growing interest in analyzing and targeting protein-protein interactions has resulted in an increased need for detailed methodologies to interrogate and monitor protein-protein interactions. To meet this need, Protein-Protein Interactions: Methods and Applications has been updated and expanded. The second edition includes core technological platforms used to study protein-protein interactions, and cutting-edge technologies that reflect recent scientific advances and the emerging focus on therapeutic discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of necessary materials and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. These well-detailed protocols describe methods for identifying protein-protein interaction partners, analyzing protein-protein interactions quantitatively and qualitatively, monitoring protein-protein interactions in live cells, and predicting and determining interaction interfaces. Authoritative and cutting-edge, Protein-Protein Interactions: Methods and Applications, Second Edition is a valuable resource that will enable readers to elucidate the mechanisms of protein-protein interactions, determine the role of these interactions in diverse biological processes, and target protein-protein interactions for therapeutic discovery. .

Contents:

Structural basis of protein-protein interactions

Quantitative analysis of protein-protein interactions

Protein-protein interaction databases

Computational prediction of protein-protein interactions.

Structure-based computational approaches for small-molecule modulation of protein-protein interactions

Targeting protein-protein interactions for drug discovery

Studying protein-protein interactions using surface plasmon resonance

Resonant waveguide grating for monitoring bimolecular interactions

Quartz microbalance technology for probing biomolecular interactions

Label-free kinetic analysis of an antibody-antigen interaction using biolayer interferometry

Characterization of protein-protein interactions by isothermal titration calorimetry

Sedimentation equilibrium studies

Detecting protein-protein interactions by gel filtration chromatography

Using light scattering to determine the stoichiometry of protein complexes

Circular dichroism (CD) analyses of protein-protein interactions

Protein-protein interaction analysis by nuclear magnetic resonance spectroscopy

Quantitative protein analysis by mass spectrometry

Using peptide arrays created by the SPOT method for defining protein-protein interactions

Fluorescence polarization assay to quantify protein-protein interactions

Förster resonance energy transfer (FRET) microscopy for monitoring biomolecular interactions

Utilizing ELISA to monitor protein-protein interactions

Glutathione-S-transferase (GST)-fusion based assays for studying protein-protein interactions

Hexahistidine (6xHis) fusion-based assays for protein-protein interactions

Studying protein-protein interactions via blot overlay/Far Western blot

Co-immunoprecipitation from transfected cells

In vivo protein cross-linking

Identification of protein-protein interactions by standard Gal4p-based yeast two-hybrid screening

Reverse two-hybrid techniques in the yeast Saccharomyces cerevisiae

MAPPIT, a mammalian two-hybrid method for in-cell detection of protein-protein interactions

Bioluminescence resonance energy transfer to detect protein-protein interactions in live cells

Mapping biochemical networks with protein fragment complementation assays

Detection of protein-protein interaction using bimolecular fluorescence complementation assay

Split-luciferase complementation assay to detect channel-protein interactions in live cells

Confocal microscopy for intracellular co-localization of proteins

Fluorescence polarization assay to quantify protein-protein interactions in an HTS format

Estrogen receptor alpha/co-activator interaction assay - TR-FRET

High content screening biosensor assay to identify disruptors of p53-hDM2 protein-protein interactions

Discovery of inhibitors of the MDM2-p53 protein-protein interaction

Biophysical methods for identifying fragment-based inhibitors of protein-protein interactions.

Other Format:

Printed edition:

ISBN:

9781493924257

Access Restriction:

Restricted for use by site license.