Nonviral Vectors for Gene Therapy : Methods and Protocols / edited by Mark A. Findeis.

Format:

Book

Contributor:

Findeis, Mark A., editor.

SpringerLink (Online service)

Series:

Methods in molecular medicine 1543-1894 ; 65.

Springer Protocols (Springer-12345)

Methods in Molecular Medicine™, 1543-1894 ; 65

Language:

English

Subjects (All):

Life sciences.

Cytology.

Life Sciences.

Cell Biology.

Local Subjects:

Life Sciences.

Cell Biology.

Physical Description:

1 online resource (XI, 399 pages).

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2001.

System Details:

text file PDF

Summary:

Nonviral gene therapy-an emerging field with great clinical promise-avoids many problems associated with viral gene therapy and offers the possibility of superior clinical effectiveness. In Nonviral Vectors for Gene Therapy: Methods and Protocols, Mark A. Findeis has assembled a panel of active researchers to present their best methods not only for preparing, handling, and characterizing gene delivery agents, but also for gene delivery. To help those preparing and characterizing gene transfer agents, the contributors examine a broad range of compounds that bind with DNA to form the compact complexes that facilitate cellular delivery-among them peptide conjugates, synthetic polymers, and lipids. They also outline specific approaches to gene transfer in vivo, including direct delivery by intratumoral injection and indirect delivery by cell-specific targeting of DNA complexes, and discuss in detail many spectroscopic techniques for characterizing nonviral gene delivery agents. Opportunities for the development and application of these novel vectors in the research lab and eventually, in the clinic, are highlighted. Comprehensive and state-of-the-art, Nonviral Vectors for Gene Therapy: Methods and Protocols illuminates for today's investigators the powerful new approaches to the creation of nonviral materials, as well as their efficacious use in delivering therapeutic genes to patients across the spectrum of human disease.

Contents:

Synthesis of Polyampholyte Comb-Type Copolymers Consisting of a Poly(L-lysine) Backbone and Hyaluronic Acid Side Chains for DNA Carrier

Cationic ?-Helical Peptides for Gene Delivery into Cells

Supramolecular Self-Assembly of Poly(ethylene glycol)-Block-Poly (L-lysine) Dendrimer with Plasmid DNA

Water-Soluble Cationic Methacrylate Polymers for Nonviral Gene Delivery

Stabilization of Polycation-DNA Complexes by Surface Modification with Hydrophilic Polymers

Use of Disulfide Cationic Lipids in Plasmid DNA Delivery

Interactions of Lipid-Oligonucleotide Conjugates with Low-Density Lipoprotein

Coupling of Nuclear Localization Signals to Plasmid DNA

Progress Toward a Synthetic Virus

Characterization of Polyampholyte Comb-Type Copolymer DNA Carriers

Methods for Studying the Formation of Polycation-DNA Complexes and Properties Useful for Gene Delivery

Characterization of DNA Condensates by Atomic Force Microscopy

Rapid and Systematic Transfer and Recovery of Large BACs/PACs into Mammalian Cells by HAEC Retrofitting

Systemic Delivery of Therapeutic Proteins by Intramuscular Injection of Plasmid DNA

Local Delivery of Therapeutic Proteins by Intratumoral Injection of Plasmid DNA-Lipid Complexes

Nonviral DNA Delivery from Polymeric Systems

Promotion of Duplex and Triplex DNA Formation by Polycation Comb-Type Copolymers

Lyophilization of Nonviral Gene Delivery Systems

Ultraviolet Absorption and Circular Dichroism Spectroscopy of Nonviral Gene Delivery Complexes

Characterization of Synthetic Gene Delivery Vectors by Infrared Spectroscopy

Characterization of Cationic Vector-Based Gene Delivery Vehicles Using Isothermal Titration and Differential Scanning Calorimetry

Light-Scattering Techniques for Characterization of Synthetic Gene Therapy Vectors

Renal Gene Therapy.

Other Format:

Printed edition:

ISBN:

9781592591398

Access Restriction:

Restricted for use by site license.