Circulating MicroRNAs : Methods and Protocols / edited by Nobuyoshi Kosaka.

Format:

Book

Contributor:

Kosaka, Nobuyoshi, editor.

SpringerLink (Online service)

Series:

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 1024.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 1024

Language:

English

Subjects (All):

Medicine.

Human genetics.

Biomedicine.

Human Genetics.

Local Subjects:

Biomedicine.

Human Genetics.

Physical Description:

1 online resource (XI, 270 pages) : 44 illustrations, 22 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press : Imprint: Humana Press, 2013.

System Details:

text file PDF

Summary:

MicroRNAs (miRNAs) are small non-coding RNAs that regulate various biological phenomena, such as development and homeostasis. The dysregulation of miRNA leads to disease progression, particularly of cancer. In Circulating MicroRNAs: Methods and Protocols, expert researchers in the field detail recent advances in the isolation, purification and analysis of circulating miRNAs from a variety of sources for research. The book is divided into three main topics. The first section involves the study of secretory miRNAs in cell-cell communication, and the second, the study of circulating miRNAs in body fluids. The last describes the novel techniques used to study circulating miRNAs. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Circulating MicroRNAs: Methods and Protocols seeks to aid scientists in dealing with the recent advances of RNAi technology from the bench to the bedside.

Contents:

Functional Analysis of Exosomal MicroRNA in Cell-Cell Communication Research

Isolation of Extracellular Nanovesicle MicroRNA from Liver Cancer Cells in Culture

Methods of Analysis of Dendritic Cell-derived Exosome-shuttle MicroRNA and its Horizontal Propagation Between Dendritic Cells

Analysis of MicroRNA and Protein Transfer by Exosomes During an Immune Synapse

Analysis of Viral MicroRNA Exchange via Exosomes In Vitro and In Vivo

Measurement of Precursor miRNA in Exosomes from Human ESC-Derived Mesenchymal Stem Cells

Analysis of the Transfer of Circulating microRNA Between Cells Mediated by Gap Junction

Isolation of Circulating MicroRNA Associated with RNA-binding Protein

Identification and Analysis of Circulating Exosomal microRNA in Human Body Fluids

Analyzing the Circulating MicroRNAs in Exosomes/Extracellular Vesicles from Serum or Plasma by qRT-PCR

Direct Serum Assay for MicroRNA in Cancer Patients

Analysis of MicroRNA Niches: Techniques to Measure Extracellular MicroRNA and Intracellular MicroRNA In Situ

Analysis of Circulating MicroRNA by Microarray in Liver Disease

Isolation of Circulating MicroRNA in Saliva

Purification of RNA From Milk Whey

Circulating MicroRNAs in the Cerebrospinal Fluid of Patients with Brain Diseases

Methods of MicroRNA Quantification in Urinary Sediment

Circulating microRNA for the Identification of Forensically Relevant Body Fluids

Identification of Prostate Cancer-associated MicroRNAs in Circulation using a Mouse Model of Disease

A Combination of Extraction Reagent and DNA Microarray that Allows for the Detection of Global MiRNA Profiles from Serum /Plasma

Nanopore Single-molecule Detection of Circulating microRNAs.

Other Format:

Printed edition:

ISBN:

9781627034531

Access Restriction:

Restricted for use by site license.