Peptide Antibodies : Methods and Protocols / edited by Gunnar Houen.

Format:

Book

Contributor:

Houen, Gunnar, 1955- editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1348.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, 1064-3745 ; 1348

Language:

English

Subjects (All):

Medicine.

Immunology.

Biomedicine.

Local Subjects:

Biomedicine.

Immunology.

Physical Description:

1 online resource (XIV, 366 pages) : 80 illustrations, 56 illustrations in color.

Edition:

First edition 2015.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2015.

System Details:

text file PDF

Summary:

This extensive volume covers basic and advanced aspects of peptide antibody production, characterization, and uses. Although peptide antibodies have been available for many years, they continue to be a field of active research and method development. For example, peptide antibodies which are dependent on specific posttranslational modifications are of great interest, such as phosphorylation, citrullination, and others, while different forms of recombinant peptide antibodies are gaining interest, notably nanobodies, single chain antibodies, TCR-like antibodies, among others. Within this volume, those areas are covered, as well as several technical and scientific advances: solid phase peptide synthesis, peptide carrier conjugation and immunization, genomics, transcriptomics, proteomics and elucidation of the molecular basis of antigen presentation and recognition by dendritic cells, macrophages, B cells and T cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Peptide Antibodies: Methods and Protocols serves as an ideal reference for researchers exploring this vital and expansive area of study.

Contents:

Peptide Antibodies: Past, Present, and Future

The Structure of Natural and Recombinant Antibodies

Prediction of Antigenic B and T Cell Epitopes Via Energy Decomposition Analysis: Description of the Web-Based Prediction Tool BEPPE

Prediction of Antibody Epitopes

Fmoc Solid-Phase Peptide Synthesis

Peptide-Carrier Conjugation

Solid Phase Peptide-Carrier Conjugation

Analysis of Peptides and Conjugates by Amino Acid Analysis

Characterization of Synthetic Peptides by Mass Spectrometry

Interpretation of Tandem Mass Spectrometry (MSMS) Spectra for Peptide Analysis

Polyclonal Peptide Antisera

Production and Screening of Monoclonal Peptide Antibodies

Production of Epitope-Specific Antibodies by Immunization with Synthetic Epitope Peptide Formulated with CpG-DNA-Liposome Complex Without Carriers

Thioredoxin-Displayed Multipeptide Immunogens

The Purification of Natural and Recombinant Peptide Antibodies by Affinity Chromatographic Strategies

Isolation of Camelid Single-Domain Antibodies Against Native Proteins Using Recombinant Multivalent Peptide Ligands

Generation of TCR-Like Antibodies Using Phage Display

Structural Characterization of Peptide Antibodies

Automated High Throughput Mapping of Linear B Cell Epitopes Using a Statistical Analysis of High Density Peptide Microarray Data

Characterization of Peptide Antibodies by Epitope Mapping Using Resin-Bound and Soluble Peptides

Screening and Characterization of Linear B-Cell Epitopes by Biotinylated Peptide Libraries

Bead-Based Peptide Arrays for Profiling the Specificity of Modification State-Specific Antibodies

Surface Plasmon Resonance Method to Evaluate Anti-Citrullinated Protein/Peptide Antibody Affinity to Citrullinated Peptides

Specificity Analysis of Histone Modification Specific Antibodies or Reading Domains on Histone Peptide Arrays

Prion-Specific Antibodies Produced in Wild-Type Mice

Immunoblotting with Peptide Antibodies: Differential Immunoreactivities Caused by Certain Amino Acid Substitutions in a Short Peptide, and Possible Effects of Differential Refolding of the Peptide on a Nitrocellulose or PVDF Membrane

Immunocytochemical and Immunohistochemical Staining with Peptide Antibodies

Designing B-Cell Epitopes for Immunotherapy and Subunit Vaccines

Enterovirus-Specific Anti-Peptide Antibodies

Therapeutic HIV Peptide Vaccine.

Other Format:

Printed edition:

ISBN:

9781493929993

Access Restriction:

Restricted for use by site license.