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Organelle Proteomics / edited by Delphine Pflieger, Jean Rossier.
Holman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
Available
- Format:
- Book
- Series:
- Methods in molecular biology 1064-3745 ; 432.
- Springer Protocols (Springer-12345)
- Methods in Molecular Biology™, 1064-3745 ; 432
- Language:
- English
- Subjects (All):
- Life sciences.
- Biochemistry.
- Proteomics.
- Cytology.
- Life Sciences.
- Biochemistry, general.
- Cell Biology.
- Local Subjects:
- Life Sciences.
- Biochemistry, general.
- Proteomics.
- Cell Biology.
- Physical Description:
- 1 online resource (XVII, 421 pages).
- Contained In:
- Springer eBooks
- Place of Publication:
- Totowa, NJ : Humana Press, 2008.
- System Details:
- text file PDF
- Summary:
- Proteomic analyses have proven to be an invaluable tool in obtaining high-throughput protein identification from low-abundance, complex biological samples. In Organelle Proteomics, detailed protocols provide step-by-step instructions to successfully study organelle proteomes by performing the purification of the various organelles present in eukaryotic cells, as well as by preparing certain sub-fractions of organelles. A series of chapters cover the whole analytical procedure of organelle characterization, from its purification starting with whole cells up to protein identification using mass spectrometry. Devoted to methods enabling a global estimate of the reliability of the protein list assigned to an organelle, Organelle Proteomics allows scientists to gain a vital and important understanding of organelle study.
- Contents:
- Analysis of Organelles by On-Line Two-Dimensional Liquid Chromatography-Tandem Mass Spectrometry
- Analysis of Organelles by On-Line Two-Dimensional Liquid Chromatography-Tandem Mass Spectrometry
- Analysis of Organelles, Vesicles, and Protein Machineries
- Purification and Proteomic Analysis of Chloroplasts and their Sub-Organellar Compartments
- Proteomic Analysis of the Stacked Golgi Complex
- Purification of Saccharomyces cerevisiae Mitochondria by Zone Electrophoresis in a Free Flow Device
- Preparation of Respiratory Chain Complexes from Saccharomyces cerevisiae Wild-Type and Mutant Mitochondria
- Mitochondrial Proteomics
- Purification and Proteomic Analysis of the Mouse Liver Mitochondrial Inner Membrane
- Subcellular Fractionation and Proteomics of Nuclear Envelopes
- Purification and Proteomic Analysis of a Nuclear-Insoluble Protein Fraction
- Preparation Methods of Human Metaphase Chromosomes for their Proteome Analysis
- Purification and Proteomic Analysis of Plant Plasma Membranes
- Protocol to Enrich and Analyze Plasma Membrane Proteins from Frozen Tissues
- Proteomic Characterization of Membrane Protein Topology
- Free Flow Isoelectric Focusing
- Use of Gas-Phase Fractionation to Increase Protein Identifications
- Purification and Proteomic Analysis of Lysosomal Integral Membrane Proteins
- Affinity Purification of Soluble Lysosomal Proteins for Mass Spectrometric Identification
- Purification and Proteomic Analysis of Synaptic Vesicles
- Purification and Proteomics Analysis of Pancreatic Zymogen Granule Membranes
- Isolation and Proteomic Analysis of Chlamydomonas Centrioles
- Purification and Proteomic Analysis of 20S Proteasomes from Human Cells
- Characterization of E. coli Ribosomal Particles
- Cellular, Genetic and Molecular Mechanisms of Plant Embryogenesis
- Assessment of Organelle Purity Using Antibodies and Specific Assays
- Identifying Bona Fide Components of an Organelle by Isotope-Coded Labeling of Subcellular Fractions
- Determination of Genuine Residents of Plant Endomembrane Organelles using Isotope Tagging and Multivariate Statistics
- Quantitative Proteomic Analysis to Profile Dynamic Changes in the Spatial Distribution of Cellular Proteins
- Use of Transcriptomic Data to Support Organelle Proteomic Analysis.
- Other Format:
- Printed edition:
- ISBN:
- 9781597450287
- Access Restriction:
- Restricted for use by site license.
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