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Basic Protein and Peptide Protocols / edited by John M. Walker.

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Holman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
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Format:
Book
Contributor:
Walker, John M., editor.
SpringerLink (Online service)
Series:
Methods in molecular biology 1064-3745 ; 32.
Springer Protocols (Springer-12345)
Methods in Molecular Biology™, 1064-3745 ; 32
Language:
English
Subjects (All):
Life sciences.
Biochemistry.
Life Sciences.
Biochemistry, general.
Local Subjects:
Life Sciences.
Biochemistry, general.
Physical Description:
1 online resource (XII, 490 pages).
Contained In:
Springer eBooks
Place of Publication:
Totowa, NJ : Humana Press, 1994.
System Details:
text file PDF
Contents:
The Lowry Method for Protein Quantitation
The Bicinchoninic Acid (BCA) Assay for Protein Quantitation
The Bradford Method for Protein Quantitation
Nondenaturing Polyacrylamide Gel Electrophoresis of Proteins
SDS Polyacrylamide Gel Electrophoresis of Proteins
Gradient SDS Polyacrylamide Gel Electrophoresis of Proteins
Acetic Acid-Urea Polyacrylamide Gel Electrophoresis of Proteins
Electrophoresis of Peptides
Isoelectric Focusing of Proteins in Ultra-Thin Polyacrylamide Gels
The In Vivo Isotopic Labeling of Proteins for Polyacrylamide Gel Electrophoresis
Two-Dimensional Polyacrylamide Gel Electrophoresis of Proteins
Two-Dimensional Polyacrylamide Gel Electrophoresis Using Immobilized pH Gradients in the First Dimension
Two-Dimensional Polyacrylamide Gel Electrophoresis Using Flat-Bed Isoelectric Focusing in the First Dimension
Quantification of Proteins on Polyacrylamide Gels (Nonradioactive)
Detection of Proteins in Polyacrylamide Gels Using an Ultrasensitive Silver Staining Technique
Identification of Glycoproteins on Nitrocellulose Membranes and Gels
The Release of Oligosaccharides from Glycoproteins
Structural Profiling of Oligosaccharides of Glycoproteins
Drying Gels
Fluorography of Polyacrylamide Gels Containing Tritium
The Electrophoretic Elution of Proteins from Polyacrylamide Gels
Preparation of Proteins from Gels for Protein Microsequencing
Comparison of Protein Primary Structures
Protein Blotting
Detection of Polypeptides on Immunoblots Using Secondary Antibodies or Protein A
Detection of Proteins on Blots Using the Avidin-Biotin System
Detection of Proteins on Protein Blots Using Chemiluminescent Systems
The Detection of Proteins on Blots Using Gold or Immunogold
Amino Acid Analysis
Molecular-Weight Estimation for Native Proteins Using Size-Exclusion High-Performance Liquid Chromatography
Peptide Purification by Reverse-Phase HPLC
Enzymatic Methods for Cleaving Proteins
Chemical Cleavage of Proteins
Chemical Modification of Proteins
The Dansyl Method for Identifying N-Terminal Amino Acids
The Dansyl-Edman Method for Peptide Sequencing
C-Terminal Sequencing of Peptides
Analysis of Cysteine Residues and Disulfide Bonds
Preparation and Characterization of Monoclonal Antibodies to Proteins and Other Cellular Components
The Raising of a Polyclonal Antiserum to a Protein
Production of Antisera to Synthetic Peptides
Production of Antibodies Using Proteins in Gel Bands
Preparation of Purified Immunoglobulin G (IgG)
Enzyme and Biotin Labeling of Antibody
Labeling of Peptides and Proteins by Radioiodination
Radioimmunoassay of Peptides and Proteins
Enzyme-Linked Immunosorbent Assay (ELISA)
Free Zone Capillary Electrophoresis.
Other Format:
Printed edition:
ISBN:
9781592595198
Access Restriction:
Restricted for use by site license.

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