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Chromosomal Mutagenesis / edited by Gregory D. Davis, Kevin J. Kayser.
Connect to full text Available online
View onlineHolman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
Available
- Format:
- Book
- Series:
- Methods in molecular biology 1064-3745 ; 435.
- Springer Protocols (Springer-12345)
- Methods in Molecular Biology, 1064-3745 ; 435
- Language:
- English
- Subjects (All):
- Life sciences.
- Human genetics.
- Molecular biology.
- Biochemistry.
- Cytology.
- Life Sciences.
- Biochemistry, general.
- Human Genetics.
- Cell Biology.
- Molecular Medicine.
- Local Subjects:
- Life Sciences.
- Biochemistry, general.
- Human Genetics.
- Cell Biology.
- Molecular Medicine.
- Physical Description:
- 1 online resource (XVI, 236 pages) : 39 illustrations, 3 illustrations in color.
- Contained In:
- Springer eBooks
- Place of Publication:
- Totowa, NJ : Humana Press, 2008.
- System Details:
- text file PDF
- Summary:
- Great disparities exist between organisms with regard to the relative ease of chromosomal mutagenesis and manipulation. In Chromosomal Mutagenesis, a team of experts provide a variety of chromosomal manipulation techniques, including insertional gene disruptions, gene knockouts, stimulated homologous recombination techniques and other novel tools, for both prokaryotic and eukaryotic organisms, and attempt to expand the genetic toolbox beyond model organisms. Following the format of the highly successful Methods in Molecular Biology™ format, each chapter offers step-by-step laboratory instructions, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Chomosomal Mutagenesis covers state-of-the-art techniques that are staged to expand, if not revolutionize, genetic analysis in the long neglected and relevant cell types.
- Contents:
- Biallelic Gene Knockouts in Chinese Hamster Ovary Cells
- Highly Proficient Gene Targeting by Homologous Recombination in the Human Pre-B Cell Line Nalm-6
- Robust Cell Line Development Using Meganucleases
- Design and Testing of Zinc Finger Nucleases for Use in Mammalian Cells
- Gene Targeting in Drosophila and Caenorhabditis elegans With Zinc-Finger Nucleases
- Orpheus Recombination
- Transposon-Mediated Mutagenesis in Somatic Cells
- Insertional Mutagenesis of the Mouse Germline With Sleeping Beauty Transposition
- Conditional Gene Trapping Using the FLEx System
- Steps Toward Targeted Insertional Mutagenesis With Class II Transposable Elements
- Targeting Integration of the Saccharomyces Ty5 Retrotransposon
- Site-Specific Chromosomal Integration Mediated by ?C31 Integrase
- Triplex-Mediated Gene Modification
- Allelic Exchange of Unmarked Mutations in Mycobacterium Tuberculosis
- Mycobacterial Recombineering
- Chromosomal Engineering of Clostridium Perfringens Using Group II Introns.
- Other Format:
- Printed edition:
- ISBN:
- 9781597452328
- Access Restriction:
- Restricted for use by site license.
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