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Hemoglobin Disorders : Molecular Methods and Protocols / edited by Ronald L. Nagel.
- Format:
- Book
- Series:
- Methods in molecular biology 1543-1894 ; 82.
- Springer Protocols (Springer-12345)
- Methods in Molecular Biology™, 1543-1894 ; 82
- Language:
- English
- Subjects (All):
- Medicine.
- Hematology.
- Medicine & Public Health.
- Local Subjects:
- Medicine & Public Health.
- Hematology.
- Physical Description:
- 1 online resource (XX, 300 pages).
- Contained In:
- Springer eBooks
- Place of Publication:
- Totowa, NJ : Humana Press, 2003.
- System Details:
- text file PDF
- Summary:
- The recent announcement that sickle-cell anemia and thalassemia have been corrected by the transplantation of stem cells bodes well for the future of gene therapy in hemoglobinopathies. In Hemoglobin Disorders: Molecular Methods and Protocols, Ronald Nagel, MD, has assembled a collection of readily reproducible techniques essential to the continued advance of our molecular understanding of these diseases. The book's richly experienced authors detail methods utilizing a wide variety of the latest analytical techniques, including X-ray crystallography, high performance liquid chromatography, electrophoresis, and nuclear magnetic resonance. Additional methods are offered for prenatal diagnostic analysis, the DNA diagnosis of hemoglobin mutations, hemoglobin fluorescence, and the semisynthesis of hemoglobin. Each protocol includes an introduction explaining the basic science, step-by-step instructions for its successful execution, notes on pitfalls to avoid, and tips on how to employ it effectively with novel systems and conditions. State-of-the-art and highly practical, Hemoglobin Disorders: Molecular Methods and Protocols reviews all the basic topics and techniques in this critically important field, and offers today's most comprehensive set of proven protocols for successful experimental and clinical work on hemoglobin diseases.
- Contents:
- X-ray Crystallography of Hemoglobins
- Analysis of Hemoglobins and Globin Chains by High-Performance Liquid Chromatography
- Purification and Molecular Analysis of Hemoglobin by High-Performance Liquid Chromatograpy
- Oxygen Equilibrium Measurements of Human Red Blood Cells
- Measurement of Rate Constants for Reactions of O2, CO, and NO with Hemoglobin
- Electrophoretic Methods for Study of Hemoglobins
- DNA Diagnosis of Hemoglobin Mutations
- Methods for Analysis of Prenatal Diagnosis
- Hemoglobin Fluorescence
- Nucleation and Crystal Growth of Hemoglobins
- Semisynthesis of Hemoglobin
- ?-Globin-like Gene Cluster Haplotypes in Hemoglobinopathies
- Transgenic Mice and Hemoglobinopathies
- Recombinant Single Globin-Chain Expression and Purification
- Nuclear Magnetic Resonance of Hemoglobins
- Solubility Measurement of the Sickle Polymer.
- Other Format:
- Printed edition:
- ISBN:
- 9781592593736
- Access Restriction:
- Restricted for use by site license.
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