Breast Cancer Research Protocols / edited by Susan A. Brooks, Adrian Harris.

Format:

Book

Contributor:

Brooks, Susan A., editor.

Harris, Adrian, 1950- editor.

SpringerLink (Online service)

Series:

Methods in molecular medicine 1543-1894 ; 120.

Springer Protocols (Springer-12345)

Methods in Molecular Medicine, 1543-1894 ; 120

Language:

English

Subjects (All):

Medicine.

Oncology.

Medicine & Public Health.

Local Subjects:

Medicine & Public Health.

Oncology.

Physical Description:

1 online resource (XIV, 518 pages).

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press : Imprint: Humana Press, 2006.

System Details:

text file PDF

Summary:

Many new technologies involving large-scale analysis of gene expression, novel treatments, and emergent combined therapy approaches have opened fresh possibilities for understanding and effectively managing breast cancer. In Breast Cancer Research Protocols, active researchers and clinicians describe in detail both well-established and cutting-edge methods for investigating breast cancer biology not only in the laboratory, but also in clinical settings. These readily reproducible techniques solve a variety of problems, ranging from how to collect, store, and prepare human breast tumor samples for analysis, to analyzing cells in vivo and in vitro. Additional chapters address the technology of handling biopsies, new methods for analyzing genes and gene expression, markers of clinical outcome and progress, analysis of tumor-derived proteins and antigens, validating targets, and investigating the biology of newly discovered genes. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Breast Cancer Research Protocols offers laboratory and clinical researchers the tools needed to illuminate breast cancer biology and advance the efficacy of its treatment today.

Contents:

Preparation of Cell and Tissue Samples

Breast Tissue Banking

Frozen Biopsy Collection and Storage

Serial Biopsies/Fine-Needle Aspirates and Their Assessment

Breast Tissue Microarrays

Preparation of Tumor Homogenates for Subsequent Preparation of Cytosols, Membrane Fractions, RNA, and DNA

Isolation of RNA From Tumor Samples

Isolation of DNA From Tumor Samples

Laser-Assisted Microdissection and Isolation of DNA and RNA

Markers of Clinical Outcome and Prognosis

Traditional and Established Indicators of Prognosis and Treatment Success

Sentinel Lymph Node Biopsy in Breast Cancer

Pathological Evaluation of Axillary Sentinel Lymph Nodes in Breast Cancer

Measurement of Estrogen Receptor Status by Immunocytochemistry in Paraffin Wax Sections

Markers of Apoptosis

Quantitative Angiogenesis in Breast Cancer

Analysis of Tumor-Derived Proteins and Antigens

Immunohistochemistry

Detection of Aberrant Glycosylation in Breast Cancer Using Lectin Histochemistry

SDS-PAGE and Western Blotting to Detect Proteins and Glycoproteins of Interest in Breast Cancer Research

Breast Cancer Proteomics Using Two-Dimensional Electrophoresis

Procedures for the Quantitative Protein Determination of Urokinase and Its Inhibitor, PAI-1, in Human Breast Cancer Tissue Extracts by ELISA

Analysis of Genes and Gene Expression in Tumor Specimens

Fluorescence In Situ Hybridization and Comparative Genomic Hybridization

Fluorescence In Situ Hybridization Assessment of c-myc Gene Amplification in Breast Tumor Tissues

Detection of HER2 Gene Amplification by Fluorescence In Situ Hybridization in Breast Cancer

In Situ Hybridization Combined With Immunohistochemistry to Localize Gene Expression

Quantitation of RNA by Ribonuclease Protection Assay

Identification of Steroid Hormone-Regulated Genes in Breast Cancer

Sequencing of the Tumor Suppressor Gene TP 53

Expression Profiling Using cDNA Microarrays

Gene Expression Analysis Using Filter cDNA Microarrays

Studying Cancer Cell Behavior in Vitro and In Vivo

Methods to Analyze the Effects of the Urokinase System on Cancer Cell Adhesion, Proliferation, Migration, and Signal Transduction Events

Phospho-Specific Antibodies as a Tool to Study In Vivo Regulation of BRCA1 After DNA Damage

Models of Hormone Resistance In Vitro and In Vivo

Generation of Genetically Modified Embryonic Stem Cells for the Development of Knockout Mouse Animal Model Systems

In Vivo Xenograft Models of Breast Cancer Metastasis

Neoadjuvant Endocrine Therapy Models

Primary Mouse Endothelial Cell Culture for Assays of Angiogenesis.

Other Format:

Printed edition:

ISBN:

9781592599691

Access Restriction:

Restricted for use by site license.