Molecular Cytogenetics : Protocols and Applications / edited by Yao-Shan Fan.

Format:

Book

Contributor:

Fan, Yao-Shan, editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 204.

Springer Protocols (Springer-12345)

Methods in Molecular Biology™, 1064-3745 ; 204

Language:

English

Subjects (All):

Medicine.

Human genetics.

Biomedicine.

Human Genetics.

Local Subjects:

Biomedicine.

Human Genetics.

Physical Description:

1 online resource (X, 414 pages).

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2003.

System Details:

text file PDF

Summary:

With the proliferation of fluorescence in situ hybridization (FISH) techniques as a powerful diagnostic tool in many areas of medicine, the variety and complexity of tests pose bewildering choices even for long-experienced laboratory technologists and physicians. In Molecular Cytogenetics: Protocols and Applications, Yao-Shan Fan and a panel of senior scientists and pioneering researchers describe in step-by-step fashion not only the leading FISH techniques, but also those molecular technologies beyond FISH now available for diagnostic services in genetics and oncology. The methods include labeling FISH probes for DNA and RNA targets, fluorescence genotyping, CGH microarray, spectral karyotyping/multicolor FISH, and primed in situ labeling. There are also techniques for multicolor fiber FISH, multi-telomere FISH, prenatal diagnosis using maternal blood, and preimplantation diagnosis. Oncological methods include simultaneous fluorescence immunophenotyping and FISH for leukemia and lymphoma, HER2 amplification in breast cancer, and CAC/PAC for cancer cytogenetics. Each easy-to-follow protocol offers first hand advice on how to obtain the best results, to avoid pitfalls, and to troubleshoot problems. Many protocols discuss possible future applications of the technology and explain its sensitivity and limitations. Cutting-edge and highly practical, Molecular Cytogenetics: Protocols and Applications offers laboratory physicians and scientists today's most comprehensive and up-to-date collection of FISH protocols for cancer diagnosis and investigation, plus an array of the emerging molecular technologies beyond FISH.

Contents:

Basic Concepts and Techniques

Molecular Cytogenetics in Medicine

Labeling Fluorescence In Situ Hybridization Probes for Genomic Targets

Labeling Fluorescence In Situ Hybridization Probes for RNA Targets

Basic FISH Techniques and Troubleshooting

Evolving Techniques and Applications

Chromosome Microdissection

Primed In Situ Labeling

Spectral Karyotyping

Multicolor FISH

Characterization of Constitutional Chromosome Abnormalities by Comparative Genomic Hybridization

Color Banding

Multicolor Fiber FISH

Multi-Telomere FISH

Fluorescence Genotyping for Screening Cryptic Telomeric Rearrangements

Microarray CGH

Special Applications in Chromosomal Disorders

Molecular Approaches for Delineating Marker Chromosomes

Interphase FISH for Prenatal Diagnosis of Common Aneuploidies

Prenatal Diagnosis of Chromosomal Abnormalities Using Maternal Blood

Preimplantation FISH Diagnosis of Aneuploidies

Microdeletion Syndromes

Molecular Detection of Uniparental Disomy

Molecular Cytogenetics in Reproductive Pathology

Special Applications in Oncology

Interphase FISH Studies of Chronic Myeloid Leukemia

Chromogenic In Situ Hybridization and FISH in Pathology

FISH Detection of HER2 Amplification in Breast Cancer

Comparative Genomic Hybridization in Cancer Investigations

Simultaneous Fluorescence Immunophenotyping and FISH on Tumor Cells

BAC Resource for Molecular Cytogenetics.

Other Format:

Printed edition:

ISBN:

9781592593002

Access Restriction:

Restricted for use by site license.