In Situ Hybridization Protocols / edited by K. H. Andy Choo.

Format:

Book

Contributor:

Choo, K. H. Andy, editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 33.

Springer Protocols (Springer-12345)

Methods in Molecular Biology™, 1064-3745 ; 33

Language:

English

Subjects (All):

Medicine.

Human genetics.

Biomedicine.

Human Genetics.

Local Subjects:

Biomedicine.

Human Genetics.

Physical Description:

1 online resource (XIX, 480 pages).

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 1995.

System Details:

text file PDF

Summary:

In situ hybridization is now recognized as an important technique in many areas of molecular biological research and its associated cli- cal studies. There is very little doubt that the applications of this te- nique will multiply in coming years since older methods are rapidly superseded by newer ones that enable the investigation of even more sophisticated biological questions and clinical problems. For the n- ice, there should thus be a sense of urgency to acquire and extend the range of this technique in order not to miss out on the large body of information that can result from its application. During the explosive growth phase of most technical innovations, such knowledge is dif- cult to acquire systematically. The aim of In Situ Hybridization Pro- cols then is to bring together a wide range of detailed laboratory protocols covering different areas of the in situ hybridization technique in order to assist such workers in rapidly advancing toward this goal. With this aim in mind, each of the protocol chapters opens with a detailed Materials section, and an easy-to-follow Methods section. Many readers will find the section headed Notes particularly useful. This s- tion contains an abundance of information and troubleshooting tips that come straight from the horse's mouth, and that are not often given in the restricted methods sections of standard research publications. For this reason, the book should also appeal to experienced workers wi- ing to add new methods to their repertoires.

Contents:

Protocols

Preparation of Human Chromosomal Painting Probes From Somatic Cell Hybrids

Fluorescent In Situ Hybridization Using Chromosome- Specific DNA Libraries

Reverse Chromosome Painting

FISH Detection on DAPI-Banded Chromosomes

Generation of Alphoid DNA Probes for Fluorescence In Situ Hybridization (FISH) Using the Polymerase Chain Reaction

In Situ Hybridization Using Synthetic Oligomers as Probes for Centromere and Telomere Repeats

Mapping Human YAC Clones by Fluorescence In Situ Hybridization Using Alu-PCR from Single Yeast Colonies

FISH of Alu-PCR-Amplified YAC Clones and Applications in Tumor Cytogenetics

Primed In Situ (PRINS) Labeling of DNA

Free Chromatin Mapping by FISH

Use of DNA- Halo Preparations for High- Resolution DNA In Situ Hybridization

In Situ Hybridization of Meiotic Prophase Chromosomes

Strand-Specific Fluorescence In Situ Hybridization for Determining Orientation and Direction of DNA Sequences

Radioactive In Situ Hybridization to Replication-Banded Chromosomes

Gene Mapping Using 3H-Labeled Heterologous Probes

Dual- label fluorescence in situ hybridization detection of individual chromosomes in sperm using ejaculated semen

In situ hybridization to drosophila polytene chromosomes

In situ hybridization to polytene chromosomes of drosophila melanogaster and other dipteran species

Microsurgical isolation of native polytene chromosomes of drosophila melanogaster for in situ molecular observation

PCR In situ hybridization

Detection of Virus Nucleic Acids by Radioactive and Nonisotopic In Situ Hybridization

Sensitive Immunocytochemical Detection of Viral Genomes

Primed In Situ (PRINS) Labeling of RNA

Hybridization Histochemistry Using Radiolabeled Oligodeoxyribonucleotide Probes

mRNA In Situ Hybridization to In Vitro Cultured Cells

Quantitative In Situ Hybridization Using Radioactive Probes in the Study of Gene Expression in Heterocellular Systems

Tissue Distribution of Gene Expression in Mammalian Development

Subcellular Location of mRNA by Electron Microscope Hybridization Histochemistry

Reveiws

Principle of Digital Imaging Microscopy

Application of In Situ Hybridization for the Detection of Virus Nucleic Acids

Positional Cloning and Multicolor In Situ Hybridization Principles and Protocols

Characterization of Constitutive Marker Chromosomes in Humans

Detection of Chromosomal Aberrations in Interphase and Metaphase Cells in Prenatal and Postnatal Studies.

Other Format:

Printed edition:

ISBN:

9781592595204

Access Restriction:

Restricted for use by site license.