PCR Detection of Microbial Pathogens / edited by Mark Wilks.

Format:

Book

Contributor:

Wilks, Mark, editor.

SpringerLink (Online service)

Series:

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 943.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 943

Language:

English

Subjects (All):

Life sciences.

Gene expression.

Microbiology.

Life Sciences.

Gene Expression.

Local Subjects:

Life Sciences.

Microbiology.

Gene Expression.

Physical Description:

1 online resource (XI, 317 pages) : 30 illustrations, 19 illustrations in color.

Edition:

Second edition 2013.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press : Imprint: Humana Press, 2013.

System Details:

text file PDF

Summary:

PCR methods for the detection of microbial pathogens have made relatively little impact in diagnostic microbiology laboratories due to the common decision to use expensive commercially produced tests rather than the cheaper alternative of developing one's own tests or introducing tests developed by other workers. PCR Detection of Microbial Pathogens, Second Edition presents alternatives to commercially produced PCR methods to detect microbial pathogens. Although most of the chapters in this book are devoted to the detection of specific pathogens, the first chapters in this book should appeal to anyone working in this field regardless of their particular interests. Although PCR tests can often be made to work with relatively little effort, it is often unclear how efficient the PCR test is, how inhibitory the specimen containing the pathogen of interest is and how the test can be quality controlled. All of which are of great importance in developing tests for diagnostic use. These topics are covered in great depth at the beginning of the book. The main part of the book is devoted to describing methods for the detection of a wide range of pathogens and from widely different specimens and situations. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, PCR Detection of Microbial Pathogens, Second Edition serves microbiologists regardless of their particular interest because, when used together with the general principles, the sheer variety of procedures provided here enables the reader to design and introduce diagnostic tests in the laboratory with confidence.

Contents:

Real-time Quantitative PCR, Pathogen Detection and MIQE

Overcoming Inhibition in Real-time Diagnostic PCR

Quality in the Molecular Microbiology Laboratory

Pre-Analytical Sample Treatment and DNA Extraction Protocols for the Detection of Bacterial Pathogens from Whole Blood

Detection of Bacterial Contamination in Platelet Concentrates using Flow Cytometry and Real-time PCR Methods

Multiplex Real-time PCR Assay for the Detection of Meticillin-Resistant Staphylococcus aureus and Panton-Valentine Leukocidin from Clinical Samples

PCR Detection of Haemophilus influenzae from Respiratory Specimens.- Rapid Detection of Atypical Respiratory Bacterial Pathogens by Real-time PCR

Use of Multiple Real-time PCR Assays for the Diagnosis of Bodetella spp in Clinical Specimens

Detection of Mycoplasma pneumoniae by Real-time PCR

Real-Time PCR Assay for the Diagnosis of Pneumocystis jirovecii Pneumonia

Rapid Identification of Mycobacteria and Rapid Detection of Drug Resistance in Mycobacterium tuberculosis in Cultured Isolates and in Respiratory Specimens

Direct Detection of Mycobaterium ulcerans in Clinical Specimens and Environmental Samples

Detection of Bartonella spp. DNA in Clinical Specimens using an Internally Controlled Real-time PCR Assay

Direct Identification of Multiple Genital Pathogens in Urine using Multiplex PCR-based Reverse Line Blot Assays

Diagnosis of Clostridium difficile Infection using Real-time PCR

Detection of Pathogenic Leptospira spp. through Real-time PCR (qPCR) Targeting the LipL32 Gene

Sensitive and Rapid Detection of Campylobacter jejuni and Campylobacter coli using Loop-mediated Isothermal Amplification

PCR Detection of Helicobacter pylori in Clinical Samples

Rapid Detection of the Escherichia coli Genospecies in Water by Conventional and Real-time PCR

Multiplex Real Time PCR (MRT-PCR) for Diarrheagenic Escherichia coli.

Other Format:

Printed edition:

ISBN:

9781603273534

Access Restriction:

Restricted for use by site license.