Free Radical and Antioxidant Protocols / edited by Donald Armstrong.

Format:

Book

Contributor:

Armstrong, Donald, editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 108.

Springer Protocols (Springer-12345)

Methods in Molecular Biology™, 1064-3745 ; 108

Language:

English

Subjects (All):

Life sciences.

Biochemistry.

Life Sciences.

Biochemistry, general.

Local Subjects:

Life Sciences.

Biochemistry, general.

Physical Description:

1 online resource (XVII, 455 pages).

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 1998.

System Details:

text file PDF

Summary:

In Free Radical and Antioxidant Protocols, seventy-six leading international authorities describe cutting-edge methodologies for quantifying free radical and antioxidant analytes in tissue and body fluids using experimental models and in vitro procedures. These user-friendly and easily reproducible techniques cover the essential tasks, including radical generating systems, direct measurement or trapping of reactive radical species and acute-phase proteins, and measurement of metabolic intermediates derived from the oxidation of lipids, proteins, and nucleic acids. There are also methods for the determination of vitamin, enzymatic, and water-soluble antioxidants, as well as of essential micronutrients and cofactors. The techniques take advantage of new instrumentation-probes, photon counting, chemiluminescence, and caged compounds, with an emphasis on HPLC-and are adaptable to a wide range of applications. Free Radical and Antioxidant Protocols provides state-of-the-art methodology and biotechnology in a convenient format for both academic and corporate biomedical scientists. The detailed, laboratory-tested free-radical assays, many of them presented here for the first time, will illuminate the study of both primary and secondary oxidative stress and contribute significantly to our understanding of the many disorders associated with this process.

Contents:

Techniques for the Measurement of Oxidative Stress

Oxygen Consumption Methods

Spin Trapping and Electron Paramagnetic Resonance Spectroscopy

In Vivo Detection of Free Radicals in Real Time by Low-Frequency Electron Paramagnetic Resonance Spectroscopy

Single Photon Counting

Measurement of Reactive Oxygen Species in Whole Blood and Mononuclear Cells Using Chemiluminescence

Assay of Phospholipid Hydroperoxides by Chemiluminescence-Based High-Performance Liquid Chromatography

Sensitive and Specific Fluorescent Probing of Oxidative Stress in Different Classes of Membrane Phospholipids in Live Cells Using Metabolically Integrated cis-Parinaric Acid

Aromatic Hydroxylation

Simple Assay for the Level of Total Lipid Peroxides in Serum or Plasma

Simple Procedure for Specific Assay of Lipid Hydroperoxides in Serum or Plasma

Antibodies Against Malondialdehyde-Modified Proteins

Oxidized LDL and Lp(a)

Oxidized and Unoxidized Fatty Acyl Esters

Synthesis of Lipid and Cholesterol Hydroperoxide Standards

Separation of Hydroxy and Hydroperoxy Polyunsaturated Fatty Acids By High-Pressure Liquid Chromatography

Products of Creatinine with Hydroxyl Radical as a Useful Marker of Oxidative Stress In Vivo

Quantitative Analysis of Peptide and Protein Changes in Ischemic Hippocampal Tissue by HPLC

Electrochemical Detection of 8-Hydroxy-2-Deoxyguanosine Levels in Cellular DNA

Nitric Oxide Synthase

Quantitation of Heme Oxygenase (HO-1) Copies in Human Tissues by Competitive RT/PCR

A Fluorescent-Based Assay for Measurement of Phospholipase A2 Activity

Autofluorescent Ceroid/Lipofuscin

In Vivo Technique for Autofluorescent Lipopigments

An Ex Vivo Erythrocyte Model for Investigating Oxidative Metabolism

Purification of Vesicular Carriers from Rat Hepatocytes by Magnetic Immunoadsorbtion

Techniques for the Measurement of Antioxidant Activity

Simultaneous Determination of Serum Retinol, Tocopherols, and Carotenoids by HPLC

EPR Measurements of Nitric Oxide-Induced Chromanoxyl Radicals of Vitamin E

Nonvitamin Plasma Antioxidants

Regulatory Antioxidant Enzymes

In Vitro Screening for Antioxidant Activity

Antioxidant Activity of Low-Density Lipoprotein

Lipoic Acid as an Antioxidant

Reduced Glutathione and Glutathione Disulfide

Analysis of Coenzyme Q10 Content in Human Plasma and Other Biological Samples

Techniques Applicable to Both Oxidative Stress and Antioxidant Activity

A Simple Luminescence Method for Detecting Lipid Peroxidation and Antioxidant Activity In Vitro

Analysis of Oxidation and Antioxidants Using Microtiter Plates

Trace Element Analysis of Biological Samples by Analytical Atomic Spectroscopy

Energy Dispersive X-Ray Microanalysis

Pro-Oxidant and Antioxidant Effects of Estrogens

Artificial Radical Generating and Scavenging Systems.

Other Format:

Printed edition:

ISBN:

9781592592548

Access Restriction:

Restricted for use by site license.