PCR Mutation Detection Protocols / edited by Bimal D. M. Theophilus, Ralph Rapley.

Format:

Book

Contributor:

Theophilus, Bimal D. M., editor.

Rapley, Ralph, editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 187.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, 1064-3745 ; 187

Language:

English

Subjects (All):

Life sciences.

Cytology.

Life Sciences.

Cell Biology.

Local Subjects:

Life Sciences.

Cell Biology.

Physical Description:

1 online resource (X, 214 pages).

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2002.

System Details:

text file PDF

Summary:

Application of DNA technology to the identification of disease-causing mutations has become widespread in recent years. PCR Mutation Detection Protocols, provides biological and clinical investigators with a comprehensive collection of new, recent, and updated PCR-based screening methods suitable for detecting the presence of both known and novel mutations. The methods cover point mutations (e.g., ASO-PCR, SSCP, DGGE, chemical cleavage), deletions (multiplex PCR, FISH, blotting), non-sense mutations (PTT), and more. The new and exciting techniques of DNA array analysis, along with such recently developed experimental methods as conformation-sensitive gel electrophoresis, are also included. Additional coverage is given to the direct use of DNA sequencing as a detection method in its own right and to the characterization of mutations previously located by other screening techniques. Each chapter explains the basic theory behind the technique and provides valuable notes essential for its successful execution. Comprehensive and highly practical, PCR Mutation Detection Protocols assures both seasoned and novice investigators access to the highly productive and readily reproducible PCR-based mutation detection methods, techniques that are laying the groundwork for many of today's major scientific and medical advances.

Contents:

Agarose and Polyacrylamide Gel Electrophoresis

Internal Labeling of DNA Probes

End-Labeling of DNA Probes

Southern Blotting of Agarose Gels by Capillary Transfer

Restriction Fragment Length Polymorphism

PCR

Allele-Specific Oligonucleotide PCR

Long-Range PCR

Analysis of Nucleotide Sequence Variations by Solid-Phase Minisequencing

Cycle Sequencing of PCR Products

Fluorescent In Situ Hybridization

The Protein Truncation Test

Mutation Detection in Factor VIII cDNA from Lymphocytes of Hemophilia A Patients by Solid Phase Fluorescent Chemical Cleavage of Mismatch

Denaturing Gradient Gel Electrophoresis

Conformation-Sensitive Gel Electrophoresis

SSCP/Heteroduplex Analysis

Cleavase® Fragment Length Polymorphism Analysis for Genotyping and Mutation Detection

Automated Genotyping Using the DNA MassArray™ Technology

An Introduction to Bioinformatics.

Other Format:

Printed edition:

ISBN:

9781592592739

Access Restriction:

Restricted for use by site license.