Next-Generation MicroRNA Expression Profiling Technology : Methods and Protocols / edited by Jian-Bing Fan.

Format:

Book

Contributor:

Fan, Jian-Bing, editor.

SpringerLink (Online service)

Series:

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 822.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 822

Language:

English

Subjects (All):

Medicine.

Human genetics.

Gene expression.

Biomedicine.

Human Genetics.

Gene Expression.

Local Subjects:

Biomedicine.

Human Genetics.

Gene Expression.

Physical Description:

1 online resource (XI, 341 pages) : 64 illustrations, 11 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2012.

System Details:

text file PDF

Summary:

The rapid pace of microRNA (miRNA) research continues to drive the advances of techniques for miRNA expression profiling, and innovative technologies that are more sensitive, specific, quantitative, and that are compatible with a wide range of biospecimens have been developed during the past few years. In Next-Generation MicroRNA Expression Profiling Technology: Methods and Protocols, expert researchers in the field contribute detailed examinations of the most current approaches being used today. This volume includes comprehensive coverage of methodologies that have been developed for miRNA profiling, as well as next-gen sequencing technology, miRNA databases, and specialized applications, such as cancer studies and miRNA-based non-invasive biomarker development. Written in the highly successful Methods in Molecular Biology™ series format, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Next-Generation MicroRNA Expression Profiling Technology: Methods and Protocols provides an ideal guide for novices and experts alike who are seeking to utilize these powerful technologies.

Contents:

Controlling miRNA Regulation in Disease

Introduction to miRNA Profiling Technologies and Cross-Platform Comparison

Stem-Loop RT-qPCR for MicroRNA Expression Profiling

Poly(T) Adaptor RT-PCR

MicroRNA In Situ Hybridization

Agilent MicroRNA Microarray Profiling System

miRNA Gene Expression Profiling Using Illumina Universal BeadChips

MicroRNA Expression Analysis Using the Affymetrix Platform

Individualized miRNA Assay Panels Using Optically Encoded Beads

Microfluidic Primer Extension Assay (MPEA)

MicroRNA Profiling Using µParaflo Microfluidic Array Technology

MicroRNA Expression Analysis Using the Illumina MicroRNA-Seq Platform

Next Generation Sequencing of miRNAs with Roche 454 GS-FLX Technology: Steps for a Successful Application

Methods for Small RNA Preparation for Digital Gene Expression Profiling by Next-Generation Sequencing

Profiling of Short RNAs Using Helicos Single-Molecule Sequencing

deepBase: Annotation and Discovery of microRNAs and Other Non-Coding RNAs from Deep Sequencing Data

PhenomiR: MicroRNAs in Human Diseases and Biological Processes

miRNA Expression Profiling: From Reference Genes to Global Mean Normalization

miRNA Data Analysis: Next-Gen Sequencing

Integrated miRNA Expression Analysis and Target Prediction

miRNAs in Human Cancer

Blood-Based miRNA Preparation for Non-Invasive Biomarker Development.

Other Format:

Printed edition:

ISBN:

9781617794278

Access Restriction:

Restricted for use by site license.