Cell Cycle Control and Dysregulation Protocols : Cyclins, Cyclin-Dependent Kinases, and Other Factors / edited by Antonio Giordano, Gaetano Romano.

Format:

Book

Contributor:

Giordano, Antonio, MD, editor.

Romano, Gaetano, 1959- editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 285.

Springer Protocols (Springer-12345)

Methods in Molecular Biology™, 1064-3745 ; 285

Language:

English

Subjects (All):

Medicine.

Immunology.

Biomedicine.

Local Subjects:

Biomedicine.

Immunology.

Physical Description:

1 online resource (XIII, 185 pages).

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2004.

System Details:

text file PDF

Summary:

The dysregulation of the normal cell cycle underlies an enormous number of pathological conditions, including the neurodegenerative disorders, all cancers, and diabetes. In Cell Cycle Control and Dysregulation Protocols: Cyclins, Cyclin-Dependent Kinases, and Other Factors, expert laboratorians detail emerging methodologies for studying the cell cycle, its kinases, and kinase inhibitors. The authors focus on issues of gene expression in vivo and in vitro, the analysis of cyclin-dependent kinase inhibitors, protein degradation mediated by the proteosome, the analysis of the transformed cell phenotype, and innovative techniques to detect apoptosis. Many of the protocols are new, such as electron microsocopy to detect apoptosis and proteosome-mediated degradation, whereas others study the interactions between protein-protein, protein-DNA, and protein-RNA. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Cell Cycle Control and Dysregulation Protocols: Cyclins, Cyclin-Dependent Kinases, and Other Factors offers pathbreaking researchers powerful tools for probing the cell cycle regulation across a wide variety of pathologies.

Contents:

Analysis of Cyclins and Cyclin-Dependent Kinases

The Biology of Cyclins and Cyclin-Dependent Protein Kinases

In Situ Immunofluorescence Analysis

Immunofluorescence Analysis Using Epitope-Tagged Proteins

Analysis of In Vivo Gene Expression Using Epitope-Tagged Proteins

Chromatin Immunoprecipitation

Protein-Deoxyribonucleic Acid Interactions Linked to Gene Expression

Assays for Cyclin-Dependent Kinase Inhibitors

Protein Degradation Via the Proteosome

Analysis of the Factors Involved in Cell Cycle Deregulation

The Transformed Phenotype

A Morphologic Approach to Detect Apoptosis Based on Electron Microscopy

Detection of Apoptotic Deoxyribonucleic Acid Break by In Situ Nick Translation

Cellular Response to Deoxyribonucleic Acid Damage

Induction of Deoxyribonucleic Acid Damage by Alkylating Agents

Induction of Deoxyribonucleic Acid Damage by ? Irradiation

Ultraviolet Irradiation of Cells

Retroviridae-Based Vectors: Protocols for Lentiviral- and Retroviral-Mediated Gene Transfer to Engineer Cell Culture Systems

Transient Production of Retroviral- and Lentiviral-Based Vectors for the Transduction of Mammalian Cells

Determination of Functional Viral Titer by Drug-Resistance Colony Assay, Expression of Green Fluorescent Protein, and ?-Galactoside Staining

Retroviral and Lentiviral Vector Titration by the Analysis of the Activity of Viral Reverse Transcriptase

Detection of Gene Expression in Subcellular Compartments

Single and Double Colloidal Gold Labeling in Postembedding Immunoelectron Microscopy

Multifluorescence Labeling and Colocalization Analyses.

Other Format:

Printed edition:

ISBN:

9781592598229

Access Restriction:

Restricted for use by site license.