Molecular Methods for Evolutionary Genetics / edited by Virginie Orgogozo, Matthew V. Rockman.

Format:

Book

Contributor:

Orgogozo, Virginie, editor.

Rockman, Matthew V., editor.

SpringerLink (Online service)

Series:

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 772.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 772

Language:

English

Subjects (All):

Medicine.

Human genetics.

Medical genetics.

Biomedicine.

Human Genetics.

Gene Function.

Local Subjects:

Biomedicine.

Human Genetics.

Gene Function.

Physical Description:

1 online resource (XVI, 503 pages) : 81 illustrations, 4 illustrations in color.

Edition:

1.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2011.

System Details:

text file PDF

Summary:

We are entering a particularly fruitful period in evolutionary genetics, as rapid technological progress transforms the investigation of genetic variation within and between species. Molecular Methods for Evolutionary Genetics is a collection of advanced molecular biology protocols and general overviews intended to represent the essential methods currently bringing evolutionary genetics to fruition. Divided into six thematic sections, this volume covers methods for characterizing genomes, diverse approaches to enrich DNA for subsets of the genome prior to sequencing, and state-of-the-art protocols for sampling genetic variation for genetic mapping studies and population genetic studies (RAD sequencing, Sequenom, microarrays, et cetera). The volume concludes by focusing on methods to study candidate genes, from obtaining their sequences and analyzing their transcripts to experimentally manipulating their activities in vivo. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Molecular Methods for Evolutionary Genetics serves as a rich resource to biologists interested in evolution, whether they be specialists or beginners in molecular biology.

Contents:

Genome Size Determination using Flow Cytometry of Propidium Iodide-stained Nuclei

Chromosome Analysis in Invertebrates and Vertebrates

Genomic Libraries. I. Construction and Screening of Fosmid Genomic Libraries

Genomic Libraries. II. Subcloning, Sequencing, and Assembling Large-insert Genomic DNA Clones

Reduced Representation Methods for Sub-genomic Enrichment and Next-generation Sequencing

Accessing the Transcriptome: How to Normalize mRNA Pools

Transcriptome Sequencing Goals, Assembly, and Assessment

Rapid Retrieval of DNA Target Sequences by Primer Extension Capture

SNP Discovery and Genotyping for Evolutionary Genetics using RAD Sequencing

DNA Microarray-based Mutation Discovery and Genotyping

Genotyping with Sequenom

Isolating Microsatellite Loci: Looking Back, Looking Ahead

Design of Custom Oligonucleotide Microarrays for Single Species or Interspecies Hybrids using Array Oligo Selector

Identification of Homologous Gene Sequences by PCR with Degenerate Primers

Characterizing cDNA Ends by Circular RACE

Identification of DNA Sequences that Flank a Known Region by Inverse PCR

Quantification of Transcript Levels with Quantitative RT-PCR

Using Pyrosequencing to Measure Allele-specific mRNA Abundance and Infer the Effects of Cis- and Trans-regulatory Differences

Whole Mount In situ Hybridization of Sectioned Tissues of Species Hybrids to Detect Cis-Regulatory Changes in Gene Expression Pattern

Identifying Fluorescently Labeled Single Molecules in Image Stacks using Machine Learning

Experimental Approaches to Evaluate the Contributions of Candidate Cis-regulatory Mutations to Phenotypic Evolution

Experimental Approaches to Evaluate the Contributions of Candidate Protein-coding Mutations to Phenotypic Evolution

Making Reporter Gene Constructs to Analyze Cis-regulatory Elements

PCR-directed In vivo Plasmid Construction using Homologous Recombination in Baker's Yeast

Production of Fosmid Genomic Libraries Optimized for Liquid Culture Recombineering and Cross-species Transgensis

Recombination-mediated Genetic Engineering of Large Genomic DNA Transgenes

Overlap Extension PCR: An Efficient Method for Transgene Construction

Gene Knockdown Analysis by Double-stranded RNA Injection.

Other Format:

Printed edition:

ISBN:

9781617792281

Access Restriction:

Restricted for use by site license.