Advanced Protocols in Oxidative Stress I / edited by Donald Armstrong.

Format:

Book

Contributor:

Armstrong, Donald, editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 477.

Springer Protocols (Springer-12345)

Methods In Molecular Biology, 1064-3745 ; 477

Language:

English

Subjects (All):

Life sciences.

Biochemistry.

Cytology.

Oxidative stress.

Life Sciences.

Biochemistry, general.

Cell Biology.

Oxidative Stress.

Local Subjects:

Life Sciences.

Biochemistry, general.

Cell Biology.

Oxidative Stress.

Physical Description:

1 online resource (XIV, 430 pages) : 100 illustrations, 1 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2008.

System Details:

text file PDF

Summary:

Protocols books specializing in measuring free radical and antioxidant biomarkers began to be published in 1998. Many of these methods are currently finding use in diagnostic medicine. Advanced Protocols in Oxidative Stress I covers the field of oxidative stress with state-of-the-art technology to utilize in research, contributed by an international panel of experts renowned for developing new procedures and methods. Included are sections on reactive oxygen and nitrogen species techniques, antioxidant technology and application, methods for analyzing gene expression, the exciting new area of oxidative stress and stem cell differentiation and specific biostatistical evaluation of biomarkers. This volume presents the current high-tech methodologies and provides a perspective on the diversity of applications in the ever-emerging field of free radical reactions and antioxidants. Due to the dynamic nature of this topic, this book will be the first of several volumes of Advanced Protocols in Oxidative Stress, all part of the highly successful Methods in Molecular Biology™ series. As part of the series, the chapters include a brief introduction to the material, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and ensuring replication of technology. Cutting-edge and convenient, Advanced Protocols in Oxidative Stress I is an ideal desk reference for scientists wishing to further this research in this exciting, unique and vital field of study.

Contents:

Reactive Oxygen and Nitrogen Techniques

Novel Designed Probes for the Characterization of Oxidative Stress in Biological Fluids, Cells, and Tissues

A Rapid and Selective Mass Spectrometric Method for the Identification of Nitrated Proteins

An Easy and Reliable Automated Method to Estimate Oxidative Stress in the Clinical Setting

Correlative Transmission Microscopy: Cytochemical Localization and Immunocytochemical Localization in Studies of Oxidative and Nitrosative Stress

Detection of Specifically Oxidized Apolipoproteins in Oxidized HDL

High Performance Liquid Chromatography/Electron Spin Resonance/Mass Spectrometry Analyses of Lipid-Derived Radicals

EPR Spin-Trapping and Nano LC MS/MS Techniques for DEPMPO/·OOH and Immunospin-Trapping with Anti-DMPO Antibody in Mitochondrial Electron Transfer System

Determination of High Mitochondrial Membrane Potential in Spermatozoa Loaded with the Mitochondrial Probe 5,5?,6,6?-Tetrachloro-1,1?,3,3?-Tetraethylbenzimidazolyl-Carbocyanine Iodide (JC-1) by Using Fluorescence-Activated Flow Cytometry

2,2,6,6-Tetramethylpiperidin-1-Oxyl Probes for Evaluating Oxidative Stress on the Cell Membrane and Mitochondria

Zymographical Techniques for Detectionof Matrix Metalloproteinases

The Use of In Vivo Microdialysis Techniques to Detect Extracellular ROS in Resting and Contracting Skeletal Muscle

Cell-Free Antibody Capture Method for Analysis of Detergent-Resistant Membrane Rafts

Determination of Acrolein by High-Voltage Capillary Electrophoresis from Oxidized Fatty Acids

Antioxidant Technology and Application

Cupric Ion Reducing Antioxidant Capacity Assay for Food Antioxidants: Vitamins, Polyphenolics, and Flavonoids in Food Extracts

Redox Property of Ribonucleotide ReductaseSmall Subunit M2 and p53R2

Antioxidant QSAR Modeling as Exemplified on Polyphenols

Immunohistochemical Staining of Cyclooxygenases with Monoclonal Antibodies

Examining the Endogenous Antioxidant Response Through Immunofluorescent Analysis of Nrf2 in Tissue

Determination of Oxidized and Reduced CoQ10 and CoQ9 in Human Plasma/Serum Using HPLC-ECD

Paraoxonases (PON1, PON2, PON3) Analyses In Vitro and In Vivo in Relation to Cardiovascular Diseases

Preparation, Characterization, and Use of Antioxidant-Liposomes

Antioxidant Activity of Biotransformed Sex Hormones Facilitated by Bacillus stearothermophilus

Biolistic Mediated Production of Transgenic Oil Palm

Preparation of a Multi-antioxidant Formulation

Gene Expression

A Functional Genomics Approach to Identify and Characterize Oxidation Resistance Genes

Genome-Wide Overexpression Screen for Activators of Antioxidant Gene Transcription

Method for Conducting Microarray Study of Oxidative Stress Induced Gene Expression

A Systems Approach Demonstrating Sphingolipid-Dependent Transcription in Stress Responses

Bioluminescence: Imaging Modality for In Vitro and In Vivo Gene Expression

Stem Cells

Reactive Oxygen Species and Upregulation of NADPH Oxidases in Mechanotransduction of Embryonic Stem Cells

Biostatistics

Pooling Data When Analyzing Biomarkers Subject to a Limit of Detection.

Other Format:

Printed edition:

ISBN:

9781603275170

Access Restriction:

Restricted for use by site license.