Immunochemical Protocols / edited by John D. Pound.

Format:

Book

Contributor:

Pound, John D., editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 80.

Springer Protocols (Springer-12345)

Methods in Molecular Biology™, 1064-3745 ; 80

Language:

English

Subjects (All):

Life sciences.

Cytology.

Life Sciences.

Cell Biology.

Local Subjects:

Life Sciences.

Cell Biology.

Physical Description:

1 online resource (XIII, 508 pages).

Edition:

Second Edition.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 1998.

System Details:

text file PDF

Summary:

This much anticipated second edition of the best selling Immunological Protocols provides a user-friendly, up-to-date handbook of reliable immunochemical techniques optimized for ready use by molecular biologists. It covers the breadth of relevant established methods-from protein blotting and immunoassays through the visualization of cellular antigens and in situ hybridization-each with their latest refinements. Protocols for the production and purification of important classes of immunochemical reagents are also provided, including "conventional" and recombinant antibodies, fusion proteins, and their various conjugates. The second edition of Immunochemical Protocols brings many previously specialized techniques within the grasp of all laboratory workers. It remains, like its predecessor, "an excellent laboratory manual and reference" and opens the door to the productive use of a new generation of immunochemical reagents with exciting possibilities.

Contents:

Production of Polyclonal Antisera

Raising Polyclonal Antibodies Using Nitrocellulose-Bound Antigen

Production and Characterization of Antibodies Against Synthetic Peptides

Preparation and Testing of Monoclonal Antibodies to Recombinant Proteins

A Simple Hollow-Fiber Bioreactor for the "In-House" Production of Monoclonal Antibodies

Screening of Monoclonal Antibodies Using Antigens Labeled with Acetylcholinesterase

Synthesis of Peptides for Use as Immunogens

Use of Proteins Blotted to Polyvinylidene Difluoride Membranes as Immunogens

Purification of Glycoproteins and Their Use as Immunogens

IgG Purification

Purification of Monoclonal Antibodies

Production of Bispecif ic and Trispecific F(ab)2 and F(ab)3 Antibody Derivatives

Preparation of Cytotoxic Antibody-Toxin Conjugates

Immunoaffinity Purification and Quantification of Antibody-Toxin Conjugates

Competitive ELISA

Epitope Mapping

Coupling of Antibodies with Biotin

Preparation of Avidin Conjugates

Enhanced Chemiluminescence Immunoassay

Nonradioactive Methods for Visualization of Protein Blots

Colloidal Gold Staining and Immunoprobing on the Same Western Blot

Erasure of Western Blots After Autoradiographic or Chemiluminescent Detection

Colloidal Gold Staining and Immunodetection in 2D Protein Mapping

Enzyme-Antienzyme Method for Immunohistochemistry

Immunohistochemical Detection of Bromodeoxyuridine-Labeled Nuclei for In Vivo Cell Kinetic Studies

Cell Kinetic Studies Using a Monoclonal Antibody to Bromodeoxyuridine

Immunohistochemical Detection of Cells in the Division Cycle Using Antibodies to Proliferating Cell Nuclear Antigen (PCNA)

Double Label Immunohistochemistry on Tissue Sections Using Alkaline Phosphatase and Peroxidase Conjugates

Preparation of Colloidal Gold Probes

Immunogold Probes in Light Microscopy

Immunogold Probes in Electron Microscopy

Electron Microscopic-Silver Enhancement for Double Labeling with Antibodies Raised in the Same Species

Flow Cytometric Analysis of Cell Surface Antigen Density

Multiple Immunofluorescence Analysis of Cells Using Flow Cytometry

Flow Cytometric Methods of Analyzing Apoptotic Cells

Flow Cytometric Detection of Proliferation-Associated Antigens, PCNA and Ki-67

Sorting of Human Peripheral Blood T-Cell Subsets Using Immunomagnetic Beads

Preparation and Use of Nonradioactive Hybridization Probes

Cellular Human and Viral DNA Detection by Nonisotopic In Situ Hybridization

Biotinylated Probes in Colony Hybridization

Nonisotopic In Situ Hybridization

Expression of Recombinant Antibody Fusion Proteins in E. coli

Expression of Antibody Fusion Proteins in Mammalian Cells

Antibody Screening of Bacteriophage ? gt-11 DNA Expression Libraries

Display of Antibody Chains on Filamentous Bacteriophage

Production of Human Fab Antibody Fragments from Phage Display Libraries

Antibodies from Phage Display Libraries as Immunochemical Reagents.

Other Format:

Printed edition:

ISBN:

9781592592579

Access Restriction:

Restricted for use by site license.