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Recombinant Protein Production in Yeast : Methods and Protocols / edited by Roslyn M. Bill.
Connect to full text Available online
View onlineHolman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
Available
- Format:
- Book
- Series:
- Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 866.
- Springer Protocols (Springer-12345)
- Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 866
- Language:
- English
- Subjects (All):
- Medicine.
- Human genetics.
- Biomedicine.
- Human Genetics.
- Local Subjects:
- Biomedicine.
- Human Genetics.
- Physical Description:
- 1 online resource (XII, 248 pages) : 47 illustrations, 6 illustrations in color.
- Contained In:
- Springer eBooks
- Place of Publication:
- Totowa, NJ : Humana Press, 2012.
- System Details:
- text file PDF
- Summary:
- In the last few years, significant advances have been made in understanding how a yeast cell responds to the stress of producing a recombinant protein, and how this information can be used to engineer improved host strains. The molecular biology of the expression vector, through the choice of promoter, tag and codon optimization of the target gene, is also a key determinant of a high-yielding protein production experiment. Recombinant Protein Production in Yeast: Methods and Protocols examines the process of preparation of expression vectors, transformation to generate high-yielding clones, optimization of experimental conditions to maximize yields, scale-up to bioreactor formats and disruption of yeast cells to enable the isolation of the recombinant protein prior to purification. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant Protein Production in Yeast: Methods and Protocols, seeks to aid scientists in adopting yeast as a protein production host.
- Other Format:
- Printed edition:
- ISBN:
- 9781617797705
- Access Restriction:
- Restricted for use by site license.
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