Cytokines and Colony Stimulating Factors : Methods and Protocols / edited by Dieter Körholz, Wieland Kiess.

Format:

Book

Contributor:

Körholz, Dieter, editor.

Kiess, W. (Wieland), editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 215.

Springer Protocols (Springer-12345)

Methods in Molecular Biology™, 1064-3745 ; 215

Language:

English

Subjects (All):

Medicine.

Immunology.

Biomedicine.

Local Subjects:

Biomedicine.

Immunology.

Physical Description:

1 online resource (XV, 478 pages).

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2003.

System Details:

text file PDF

Summary:

Enormous effort has been spent developing many new laboratory techniques for studying immune cells and the immune system, not only to increase fundamental understanding, but also to procure therapeutic success in a clinical setting. In Cytokines and Colony Stimulating Factors: Methods and Protocols, leading laboratory experts describe for the novice investigator a host of novel technologies and molecular techniques specifically designed to study cellular immunology and promote its gene therapy applications. Presented in step-by-step detail to ensure ready reproducibility, these protocols range from flow cytometric techniques to detect cytokines and growth factors in different specimens, to methods for generating and expanding dendritic cells and hematopoietic progenitors from different origins. Each proven method has been fine-tuned for experimental successs by the investigator who developed it, and includes notes on troubleshooting and avoiding potential pitfalls. Although the protocols require little previous experience, even highly skilled researchers will augment their experimental range with many novel timesaving techniques. Cutting-edge and highly practical, Cytokines and Colony Stimulating Factors: Methods and Protocols provides researchers a carefully evaluated set of powerful tools for successful experimental research on cytokines and their role in gene therapy.

Contents:

Primary Immunodeficiencies Caused by Defects of Cytokines and Cytokine Receptors

Detection Assays for Cytokines and Growth Factors

Intracellular Detection of T-Cell Cytokines

Analysis of Cytokine Profiles in Human Skin

Intracytoplasmic Detection of Proinflammatory Cytokines and Chemokines in Monocytes by Flow Cytometry

Flow-Cytometric Immune Function Methodology for Human Peripheral Blood Dendritic Cells

Evaluation of the Frequency of Virus-Specific CD8+T Cells by Cytokine Flow Cytometry

Detection of Common Cytokine and Colony Stimulating Factor Gene Polymorphisms

Apoptosis Induction by TRAIL

Angiogenic Cytokines

Experimental Strategies for Combined Suicide and Immune Cancer Gene Therapy

Gene Therapy with Plasmids Encoding Cytokine- or Cytokine Receptor-IgG Chimeric Proteins

Interferon-? Gene Therapy for the Treatment of Arthritis

Gene Gun-Based In Vivo Gene Transfer

Purification of the Eucaryotic Heat-Shock Proteins Hsp70 and gp96

Genetic Engineering of a Recombinant Fusion Protein Possessing an Antitumor Antibody Fragment and a TNF-? Moiety

Genetic Engineering of Dendritic Cells by Adenovirus-Mediated TNF-? Gene Transfer

Retroviral Transfer of T-Cell Receptor Genes into Human Peripheral Blood Lymphocytes

Gene Transfer to Articular Chondrocytes with Recombinant Adenovirus

Gene Therapy of X-Linked Severe Combined Immunodeficiency

Gene Transfer for Generation of Tumor and Leukemia Vaccines

Cytokine Gene Delivery into the Central Nervous System Using Intrathecally Injected Nonreplicative Viral Vectors

Ex Vivo Cell Expansion

In Vitro Regulation of Colony Stimulating Factor-Mediated Hematopoiesis in Healthy Individuals and Patients with Different Types of Myeloproliferative Disease

In Vitro Generation of Dendritic Cells from Cord Blood CD34+Hematopoietic Progenitors Cells

Generation of TransgenicT Cells from Human CD34+Cord Blood Cells

Ex Vivo Expansion of Umbilical Cord Blood Cells on Feeder Layers

Ex Vivo Production of Cord Blood CD34+Derived Myeloid Precursors After Serum-Free Static Culture

27 Ex Vivo Expansion of Hematopoietic Stem Cells

28 Large-Scale Ex Vivo Expansion of Human Megakaryocytes for Clinical Use

Cytokine-Mediated Expansion of Human NOD-SCID-Repopulating Cells

Maturation and Differentiation of Dendritic Cells

Differentiation of Human Antigen-Presenting Dendritic Cells from CD34+Hematopoietic Stem Cells In Vitro

Dendritic Cell Development from Mobilized Peripheral Blood CD34+Cells

In Vitro Maturation of Dendritic Cells from Blood Progenitors

Generation of Human Type 1- and Type 2-Polarized Dendritic Cells from Peripheral Blood

Preparation of Human Dendritic Cells for Tumor Vaccination

Generation of Leukemic Dendritic Cells from Patients with Acute Myeloid Leukemia.

Other Format:

Printed edition:

ISBN:

9781592593453

Access Restriction:

Restricted for use by site license.