Liposome Methods and Protocols / edited by Subhash C. Basu, Manju Basu.

Format:

Book

Contributor:

Basu, Subhash C., editor.

Basu, Manju, editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 199.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, 1064-3745 ; 199

Language:

English

Subjects (All):

Pharmacy.

Local Subjects:

Pharmacy.

Physical Description:

1 online resource (XII, 252 pages).

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2002.

System Details:

text file PDF

Summary:

In vitro utilization of liposomes holds great potential as a powerful tool for drug targeting, gene transport across plasma and nuclear membranes, and enzyme therapy for patients with genetic disorders. In Liposome Methods and Protocols, a panel of skilled researchers describes in step-by-step detail their best laboratory techniques for elucidating liposome biology and realizing this promise. With these readily reproducible methods, investigators can illuminate such critical questions as the attachment of liposomes to cell surfaces, the permeation of liposomes through the plasma membranes, and the stability of liposomes in cellular and nuclear matrices. Each detailed protocol is presented by an investigator who is intimately familiar with its problems and capabilities, and includes a detailed list of materials to be used, troubleshooting tips straight from the benchtop, and notes on pitfalls to avoid. The emphasis is on clearly presenting the technical steps critical to experimental success that are too often omitted from methods published in the primary literature. State-of-the-art and highly practical, Liposome Methods and Protocols provides an indispensable collection of proven techniques and references for researchers across many fields, including glycoproteins, glycolipids, glycosyltransferases, drug transport, viral transport, antibody delivery, synthetic peptide delivery to cells, and protease delivery.

Contents:

Physical Properties

Preparation, Isolation, and Characterization of Liposomes Containing Natural and Synthetic Lipids

Preparation and Use of Liposomes for the Study of Sphingolipid Segregation in Membrane Model Systems

Liposome Fusion/Modulation

Peptide-Induced Fusion of Liposomes

Liposomes

Lipids in Viral Fusion

Application of Liposomes

Liposome-Mediated, Fluorescence-Based Studies of Sphingolipid Metabolism in Intact Cells

Micelles and Liposomes in Metabolic Enzyme and Glycolipid Glycosyltransferase Assays

Liposomes and Phospholipid Binding Proteins in Glycoprotein Biosynthesis

Therapeutic Uses of Antioxidant Liposomes

Targeted Gene Delivery by Virosomes*

Liposomes Containing Ligands

Preparation and Characterization of Glycolipid-Bearing Multilamellar and Unilamellar Liposomes

Use of Liposomes Containing Carbohydrates for Production of Monoclonal Antibodies

Inhibition of Tumor Metastasis by Liposomes Containing Glyco-Replica Peptides

Use of Phospholipid Bilayers and Monolayers in Binding Studies of Vitamin K-Dependent Blood Coagulation Proteins.

Other Format:

Printed edition:

ISBN:

9781592591756

Access Restriction:

Restricted for use by site license.