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PCR Primer Design / edited by Chhandak Basu.
Holman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
Available
- Format:
- Book
- Series:
- Methods in molecular biology 1064-3745 ; 1275.
- Springer Protocols (Springer-12345)
- Methods in Molecular Biology, 1064-3745 ; 1275
- Language:
- English
- Subjects (All):
- Medicine.
- Human genetics.
- Biomedicine.
- Human Genetics.
- Local Subjects:
- Biomedicine.
- Human Genetics.
- Physical Description:
- 1 online resource (XII, 216 pages) : 73 illustrations, 45 illustrations in color.
- Edition:
- Second edition 2015.
- Contained In:
- Springer eBooks
- Place of Publication:
- New York, NY : Springer New York : Imprint: Humana Press, 2015.
- System Details:
- text file PDF
- Summary:
- This volume provides an overview on design PCR primers for successful DNA amplification. Chapters focus on primer design strategies for quantitative PCR, in silico PCR primer design , and primer design using software. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, PCR Primer Design, Second Edition seeks to aid molecular biology students, researchers, professors and PCR enthusiasts.
- Contents:
- Fast Masking Of Repeated Primer Binding Sites In Eukaryotic Genomes
- Primer Design for PCR Reactions in Forensic Biology
- Design of Primers and Probes For Quantitative Real-Time PCR Methods
- Large-scale Nucleotide Sequence Alignment And Sequence Variability Assessment To Identify the Evolutionarily Highly Conserved Regions For Universal Screening PCR Assay Design: An Example Of Influenza A Virus
- Low-concentration Initiator Primers Improve The Amplification Of Gene Targets With High Sequence Variability
- Multiplex PCR Primer Design for Simultaneous Detection of Multiple Pathogens
- Degenerate Primer Design for Highly Variable Genomes
- Allele-specific Real Time Polymerase Chain Reaction As A Tool For Urate Transporter 1 Mutation Detection
- MultiPLX: Automatic Grouping And Evaluation Of PCR Primers
- In silico PCR Primer Designing and Validation
- Primer Design using Primer Express® for SYBR Green Based Quantitative PCR
- Designing Primers for SNaPshot Technique
- Rapid and Simple Method of qPCR Primer Design
- PRIMEGENSw3: A Web-based Tool for High-throughput Primer and Probe Design
- Selecting Specific PCR Primers with MFEprimer.
- Other Format:
- Printed edition:
- ISBN:
- 9781493923656
- Access Restriction:
- Restricted for use by site license.
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