The LINC Complex : Methods and Protocols / edited by Gregg G. Gundersen, Howard J. Worman.

Format:

Book

Contributor:

Gundersen, Gregg G., editor.

Worman, Howard J., editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1840.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, 1064-3745 ; 1840

Language:

English

Subjects (All):

Cytology.

Cell Biology.

Local Subjects:

Cell Biology.

Physical Description:

1 online resource (XIV, 338 pages) : 61 illustrations, 41 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2018.

System Details:

text file PDF

Summary:

This volume describes research methodologies and approaches used to study the Linker of Nucleoskeleton and Cytoskeleton (LINC) complex and its cellular functions. Chapters detail structural and biochemical analysis of LINC complexes, mechanical aspects of the LINC complex, analysis of the LINC complex in model systems and development, and LINC complex in mammalian tissue, organs, and disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The LINC Complex: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Contents:

EM Tomography of LINC Complexes

Recombinant Purification of the Periplasmic Portion of the LINC Complex

Analysis of High Molecular Weight Isoforms of Nesprin-1 and Nesprin-2 with Vertical Agarose Gel Electrophoresis

Analysis of Nesprin-2 Interaction with its Binding Partners and Actin

Interactions of Nesprin-4-containing LINC Complexes in Outer Hair Cells Explored by BioID

Using Nesprin Tension Sensors to Measure Force on the LINC Complex

Analyzing Mechanotransduction through the LINC Complex in Isolated Nuclei

Direct Force Probe for Nuclear Mechanics

Centrifugal Displacement of Nuclei in Adherent Cells to Study LINC Complex Dependent Mechanisms of Homeostatic Nuclear Positioning

Assembly and Use of a Microfluidic Device to Study Cell Migration in Confined Environments

Investigating LINC Complex Protein Homo-oligomerization in the Nuclear Envelopes of Living Cells Using Fluorescence Fluctuation Spectroscopy

Functional Analysis of the Yeast LINC Complex Using Fluctuation Spectroscopy and Super Resolution Imaging

Genetic Analysis of Nuclear Migration and Anchorage to Study LINC Complexes During Development of Caenorhabditis elegans

High-resolution Imaging Methods to Analyze LINC Complex Function During Drosophila Muscle Development

Computational Methods for Studying the Plant Nucleus

Investigation of Nuclear Periphery Protein Interactions in Plants Using the Membrane Yeast Two-Hybrid (MbY2H) System

Immunolabeling Protocols for Studying Meiosis in Plant Mutants Defective for Nuclear Envelope Components

Generation and Analysis of Striated Muscle Selective LINC Complex Protein Mutant Mice

An in vitro System to Measure the Positioning, Stiffness and Rupture of the Nucleus in Skeletal Muscle

Functional Analysis of LINC Complexes in Skin

Detection of SUN1 Splicing Variants at the mRNA and Protein Levels in Cancer

Next-generation Sequencing and Mutational Analysis: Implications for Genes Encoding LINC Complex Proteins.

Other Format:

Printed edition:

ISBN:

9781493986910

Access Restriction:

Restricted for use by site license.