Kinase Inhibitors : Methods and Protocols / edited by Bernhard Kuster.

Format:

Book

Contributor:

Kuster, Bernhard, editor.

SpringerLink (Online service)

Series:

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 795.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 795

Language:

English

Subjects (All):

Medicine.

Pharmacology.

Biomedicine.

Pharmacology/Toxicology.

Local Subjects:

Biomedicine.

Pharmacology/Toxicology.

Physical Description:

1 online resource (XI, 256 pages) : 47 illustrations, 11 illustrations in color.

Edition:

1.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2012.

System Details:

text file PDF

Summary:

Protein and lipid kinases are often the master regulators of cell signaling in eukaryotic systems. The human genome codes for more than 500 of these enzymes and their misregulation has been shown to be involved in the onset and progression of many diseases including cancer and inflammation. Therefore, small molecule kinase inhibitors have become important research tools for the elucidation of the many biological roles of kinases and their mechanisms of action. Kinase inhibitors thus also contribute significantly to the drug pipelines of the pharmaceutical and biotechnology industries and to the growing need to treat cancer and inflammation. In Kinase Inhibitors: Methods and Protocols, experts in kinase biology, drug discovery, and clinical research present a series of exemplary methods that can be used to address the many challenges facing scientists in the discovery and development of kinase inhibitors both for research and clinical use. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Kinase Inhibitors: Methods and Protocols aims to provide scientists with modern and relevant methods to accelerate or strengthen their research and drug discovery programs through the utilization of these vital regulators.

Contents:

Targeting Cancer with Small Molecular Weight Kinase Inhibitors

Small Molecule Protein and Lipid Kinase Inhibitors in Inflammation and Specific Models for their Evaluation

Measuring the Activity of Leucine Rich Repeat Kinase 2 (LRRK2) - A Kinase Involved in Parkinson's Disease

Measuring PI3K Lipid Kinase Activity

A Fluorescence Polarization Assay for the Discovery of Inhibitors of the Polo-box Domain of Polo-like Kinase 1

Assessment of Hepatotoxicity Potential of Drug Candidate Molecules Including Kinase Inhibitors by Hepatocyte Imaging Assay Technology and Bile Flux Imaging Assay Technology

Kinase Inhibitor Selectivity Profiling using Differential Scanning Fluorimetry

Chemoproteomic Characterization of Protein Kinase Inhibitors using Immobilized ATP

Proteome-wide identification of staurosporine-binding kinases using Capture Compound Mass Spectrometry

Affinity Purification of Proteins Binding to Kinase Inhibitors Immobilised on Self Assembling Monolayers

Kinase Inhibitor Profiling using Chemoproteomics

Covalent Crosslinking of Kinases with Their Corresponding Peptide Substrates

Receptor Tyrosine Kinase Inhibitor Profiling using Bead-based Multiplex Sandwich Immunoassays

Monitoring Phosphoproteomic Response To Targeted Kinase Inhibitors Using Reverse-Phase Protein Microarrays

Measuring Phosphorylation Specific Changes in Response to Kinase Inhibitors in Mammalian Cells Using Quantitative Proteomics

Investigation of Acquired Resistance to EGFR-Targeted Therapies in Lung Cancer Using cDNA Microarrays.

Other Format:

Printed edition:

ISBN:

9781617793370

Access Restriction:

Restricted for use by site license.