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Adipose Tissue Protocols / edited by Kaiping Yang.
Holman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
Available
- Format:
- Book
- Series:
- Methods in molecular biology 1064-3745 ; 456.
- Springer Protocols (Springer-12345)
- Methods in Molecular Biology™, 1064-3745 ; 456
- Language:
- English
- Subjects (All):
- Life sciences.
- Human physiology.
- Molecular biology.
- Endocrinology.
- Cytology.
- Life Sciences.
- Cell Biology.
- Human Physiology.
- Molecular Medicine.
- Local Subjects:
- Life Sciences.
- Cell Biology.
- Human Physiology.
- Endocrinology.
- Molecular Medicine.
- Physical Description:
- 1 online resource (XVI, 336 pages) : 61 illustrations, 9 illustrations in color.
- Contained In:
- Springer eBooks
- Place of Publication:
- Totowa, NJ : Humana Press, 2008.
- System Details:
- text file PDF
- Summary:
- Since the publication of the first edition, the techniques and experimental approaches employed in the study of adipose tissue have undergone radical expansion and revision. In Adipose Tissue Protocols: Second Edition, several updated chapters are combined with many new contributions from internationally recognized experts covering both conventional and state-of-the-art methodologies as well as whole animal, cellular and molecular approaches which have emerged in or been extended to adipose tissue research over the past few years. As a volume in the highly sucessful Methods in Molecular Biology™ series, each chapter includes an introduction to its subject matter, a list of necessary materials, step-by-step, readily reproducible laboratory protocols, and a Notes section, which provides invaluable tips and tricks to safeguard against potential mishaps and pitfalls. Cutting-edge and comprehensive, Adipose Tissue Protocols: Second Edition is an essential manual for newcomers to this burgeoning area as well as a valuable addition to the laboratories and offices of more experienced researchers.
- Contents:
- Overview of Adipose Tissue and Its Role in Obesity and Metabolic Disorders
- Choosing an Adipose Tissue Depot for Sampling
- Application of Imaging and Other Noninvasive Techniques in Determining Adipose Tissue Mass
- Generation of Adipose Tissue-Specific Transgenic Mouse Models
- Angiogenesis in Adipose Tissue
- Adipose Organ Nerves Revealed by Immunohistochemistry#
- Human Adipose Tissue Blood Flow and Micromanipulation of Human Subcutaneous Blood Flow
- Studies of Thermogenesis and Mitochondrial Function in Adipose Tissues
- Application of Lipidomics and Metabolomics to the Study of Adipose Tissue
- Applications of Proteomics to the Study of Adipose Tissue
- Application of DNA Microarray to the Study of Human Adipose Tissue/Cells
- Differentiation of Adipose Stem Cells
- Methods That Resolve Different Contributions of Clonal Expansion to Adipogenesis in 3T3-L1 and C3H10T1/2 Cells
- Explant Cultures of White Adipose Tissue
- Isolation and Culture of Preadipocytes from Rodent White Adipose Tissue
- Flow Cytometry on the Stromal-Vascular Fraction of White Adipose Tissue
- Application of Electrophoretic Mobility Shift Assay and Chromatin Immunoprecipitation in the Study of Transcription in Adipose Cells
- Application of RNA Interference Techniques to Adipose Cell Cultures
- RNA Isolation and Real-Time Quantitative RT-PCR
- Study of Adipose Tissue Gene Expression by In Situ Hybridization
- Application of Immunocytochemistry and Immunofluorescence Techniques to Adipose Tissue and Cell Cultures
- Determination of Lipolysis in Isolated Primary Adipocytes
- Study of Glucose Uptake in Adipose Cells
- Measurement of Phosphoinositide 3-Kinase and Its Products to Study Adipogenic Signal Transduction.
- Other Format:
- Printed edition:
- ISBN:
- 9781597452458
- Access Restriction:
- Restricted for use by site license.
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