Bacteriophages : Methods and Protocols, Volume 2 Molecular and Applied Aspects / edited by Martha R.J. Clokie, Andrew M. Kropinski.

Format:

Book

Contributor:

Clokie, Martha R. J., editor.

Kropinski, Andrew M. (Andrew Maitland Boleslaw), 1943- editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 502.

Springer Protocols (Springer-12345)

Methods in Molecular Biology™, 1064-3745 ; 502

Language:

English

Subjects (All):

Life sciences.

Diagnosis, Laboratory.

Microbiology.

Bacteriology.

Life Sciences.

Laboratory Medicine.

Local Subjects:

Life Sciences.

Bacteriology.

Microbiology.

Laboratory Medicine.

Physical Description:

1 online resource (XXII, 373 pages).

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2009.

System Details:

text file PDF

Summary:

Ranging from the evolution of pathogenicity to oceanic carbon cycling, the many and varied roles that bacteriophages play in microbial ecology and evolution have inspired increased interest within the scientific community. Bacteriophages: Methods and Protocols pulls together the vast body of knowledge and expertise from top international bacteriophage researchers to provide both classical and state-of-the-art molecular techniques. With its well-organized modular design, Volume 2: Molecular and Applied Aspects examines a multitude of topics, including the bacteriophage genomics, metagenomics, transcriptomics, and proteomics, along with applied bacteriophage biology. Written in the highly successful Methods in Molecular Biology™ series format, chapters consist of brief introductions to the subject, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and a Notes section which details tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Bacteriophages: Methods and Protocols is a valuable reference for experienced bacteriophage researchers as well as an easily accessible introduction for newcomers to the subject.

Contents:

Bacteriophage Genomics

Preparation of Bacteriophage Lysates and Pure DNA

Approaches to the Compositional Analysis of DNA

Determination of Bacteriophage Genome Size by Pulsed-Field Gel Electrophoresis

Preparation of a Phage DNA Fragment Library for Whole Genome Shotgun Sequencing

PCR and Partial Sequencing of Bacteriophage Genomes

In Sffamily Identification of Genes in Bacteriophage DNA

Determining DNA Packaging Strategy by Analysis of the Termini of the Chromosomes in Tailed-Bacteriophage Virions

In silico Characterization of DNA Motifs with Particular Reference to Promoters and Terminators

Molecular Phylogenetics: Testing Evolutionary Hypotheses

Bacteriophage Transcriptomics and Proteomics

Preparation of RNA from Bacteria Infected with Bacteriophages: A Case Study from the Marine Unicellular Synechococcus sp. WH7803 Infected by Phage S-PM2

Quantification of Host and Phage mRNA Expression During Infection Using Real-Time PCR

Oligonucleotide Microarrays for Bacteriophage Expression Studies

Purification of Bacteriophages and SDS-PAGE Analysis of Phage Structural Proteins from Ghost Particles

Phage Proteomics: Applications of Mass Spectrometry

Community Bacteriophage Approaches

Isolation Independent Methods of Characterizing Phage Communities 1: Strain Typing Using Fingerprinting Methods

Isolation Independent Methods of Characterizing Phage Communities 2: Characterizing a Metagenome

Applied Aspects Of Bacteriophage Biology

Phage Typing

A Genetic Screen to Identify Bacteriophage Lysins

General M13 Phage Display: M13 Phage Display in Identification and Characterization of Protein-Protein Interactions

Isolation of Monoclonal Antibody Fragments from Phage Display Libraries

Internet Resources of Interest to Bacteriophage Workers.

Other Format:

Printed edition:

ISBN:

9781603275651

Access Restriction:

Restricted for use by site license.