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G-Quadruplex DNA : Methods and Protocols / edited by Peter Baumann.
Holman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
Available
- Format:
- Book
- Series:
- Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 608.
- Springer Protocols (Springer-12345)
- Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 608
- Language:
- English
- Subjects (All):
- Life sciences.
- Human genetics.
- Biochemistry.
- Cytology.
- Life Sciences.
- Biochemistry, general.
- Cell Biology.
- Human Genetics.
- Local Subjects:
- Life Sciences.
- Biochemistry, general.
- Cell Biology.
- Human Genetics.
- Physical Description:
- 1 online resource (XII, 276 pages) : 65 illustrations, 2 illustrations in color.
- Contained In:
- Springer eBooks
- Place of Publication:
- Totowa, NJ : Humana Press, 2010.
- System Details:
- text file PDF
- Summary:
- Recent work has revealed that stabilizing G-quadruplexes in telomeric DNA inhibits telomerase activity, providing impetus for the development of G-quartet-interacting drugs, while G-quartet-containing oligonucleotides have been recognized as a potent class of aptamers effective against STAT3 and other transcription factors implicated in oncogenesis, proving these guanine-quartets to be a vital and rich area for future study. In G-Quadruplex DNA: Methods and Protocols, experts in the field present a collection of detailed techniques for studying G-quartet formation, dynamics, and molecular recognition. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, G-Quadruplex DNA: Methods and Protocols promises to be a useful resource for those familiar with G-quartets as well as an easy entry point for those researchers from diverse fields who are just developing an interest in the exciting implications of G-quadruplex DNA.
- Contents:
- G-Quadruplexes: From Guanine Gels to Chemotherapeutics
- Molecular Modeling and Simulation of G-Quadruplexes and Quadruplex-Ligand Complexes
- Computational Approaches to the Detection and Analysis of Sequences with Intramolecular G-Quadruplex Forming Potential
- Preparation of G-Quartet Structures and Detection by Native Gel Electrophoresis
- Biochemical Techniques for the Characterization of G-Quadruplex Structures: EMSA, DMS Footprinting, and DNA Polymerase Stop Assay
- Real-Time Observation of G-Quadruplex Dynamics Using Single-Molecule FRET Microscopy
- Sedimentation Velocity Ultracentrifugation Analysis for Hydrodynamic Characterization of G-Quadruplex Structures
- 2-Aminopurine as a Probe for Quadruplex Loop Structures
- Assessing DNA Structures with 125I Radioprobing
- Monitoring the Temperature Unfolding of G-Quadruplexes by UV and Circular Dichroism Spectroscopies and Calorimetry Techniques
- Probing Telomeric G-Quadruplex DNA Structures in Cells with In Vitro Generated Single-Chain Antibody Fragments
- Detection of G-Quadruplexes in Cells and Investigation of G-Quadruplex Structure of d(T2AG3)4 in K+ Solution by a Carbazole Derivative: BMVC
- Isolation of G-Quadruplex DNA Using NMM-Sepharose Affinity Chromatography
- Quantifying Interactions Between G-Quadruplex DNA and Transition-Metal Complexes
- G4-FID: A Fluorescent DNA Probe Displacement Assay for Rapid Evaluation of Quadruplex Ligands.
- Other Format:
- Printed edition:
- ISBN:
- 9781597453639
- Access Restriction:
- Restricted for use by site license.
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