Cell Imaging Techniques : Methods and Protocols / edited by Douglas J. Taatjes, Brooke T. Mossman.

Format:

Book

Contributor:

Taatjes, Douglas J., editor.

Mossman, Brooke T., 1947- editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 319.

Springer Protocols (Springer-12345)

Methods in Molecular Biology™, 1064-3745 ; 319

Language:

English

Subjects (All):

Medicine.

Human anatomy.

Biochemistry.

Cytology.

Medicine & Public Health.

Anatomy.

Cell Biology.

Biochemistry, general.

Local Subjects:

Medicine & Public Health.

Anatomy.

Cell Biology.

Biochemistry, general.

Physical Description:

1 online resource (XIV, 490 pages) : 212 illustrations.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2006.

System Details:

text file PDF

Summary:

Cell imaging methodologies have now become essential research tools for a variety of disciplines that traditionally had not relied on them. In Cell Imaging Techniques: Methods and Protocols, distinguished international researchers describe in detail their state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complementary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Timely and highly practical, Cell Imaging Techniques: Methods and Protocols provides researchers and clinicians with a richly useful guide to selecting and performing the best imaging method from a bewildering variety of microscopy-based techniques.

Contents:

Molecular Beacons

Second-Harmonic Imaging of Collagen

Visualizing Calcium Signaling in Cells by Digitized Wide-Field and Confocal Fluorescent Microscopy

Multifluorescence Labeling Techniques and Confocal Laser Scanning Microscopy on Lung Tissue

Evaluation of Confocal Microscopy System Performance

Quantitative Analysis of Atherosclerotic Lesion Composition in Mice

Applications of Microscopy to Genetic Therapy of Cystic Fibrosis and Other Human Diseases

Laser Scanning Cytometry

Near-Clinical Applications of Laser Scanning Cytometry

Laser Capture Microdissection

Analysis of Asbestos-Induced Gene Expression Changes in Bronchiolar Epithelial Cells Using Laser Capture Microdissection and Quantitative Reverse Transcriptase-Polymerase Chain Reaction

New Approaches to Fluorescence In Situ Hybridization

Microarray Image Scanning

Near-Field Scanning Optical Microscopy in Cell Biology and Cytogenetics

Porosome

Secretory Vesicle Swelling by Atomic Force Microscopy

Imaging and Probing Cell Mechanical Properties With the Atomic Force Microscope

Reflection Contrast Microscopy

Three-Dimensional Analysis of Single Particles by Electron Microscopy

Three-Dimensional Reconstruction of Single Particles in Electron Microscopy

A New Microbiopsy System Enables Rapid Preparation of Tissue for High-Pressure Freezing.

Other Format:

Printed edition:

ISBN:

9781592599936

Access Restriction:

Restricted for use by site license.