RNA Remodeling Proteins : Methods and Protocols / edited by Marc Boudvillain.

Format:

Book

Contributor:

Boudvillain, Marc, editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1259.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, 1064-3745 ; 1259

Language:

English

Subjects (All):

Life sciences.

Proteins.

Life Sciences.

Protein Science.

Protein Structure.

Local Subjects:

Life Sciences.

Protein Science.

Protein Structure.

Physical Description:

1 online resource (XIV, 470 pages) : 84 illustrations, 40 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2015.

System Details:

text file PDF

Summary:

This volume provides readers with current methods to study RNA remodeling proteins. The methods, ranging from basic to complex, help the scientific community understand the role and fate of RNA species in cells, and their structures and interactions with other biomolecules. The book begins with two introductory chapters, followed by chapters where readers will find procedures to identify RNA remodeling proteins and their cofactors, physiological RNA targets and biological functions, and complex molecular mechanisms of action using purified components. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, RNA Remodeling Proteins: Methods and Protocols seeks to aid scientists in the further study of this ever evolving field of proteins and mechanisms.

Contents:

Key Points to Consider When Studying RNA Remodeling by Proteins

Happy Birthday: 25 years of DEAD-box Proteins

In vivo Cross-linking Followed by polyA Enrichment to Identify Yeast mRNA Binding Proteins

Dynamics of the Spb4 Interactome Monitored by Affinity Purification

Using EMOTE to Map the Exact 5'-ends of Processed RNA on a Transcriptome-wide Scale

Cellular Localization of RNA Degradation and Processing Components in Escherichia coli

Recombineering Applications for the Mutational Analysis of Bacterial RNA-binding Proteins and Their Sites of Action

Determination of RNA Chaperone Activity Using an Escherichia coli Mutant

Biochemical Characterization of G4 Quadruplex Telomerase RNA Unwinding by the RNA Helicase RHAU

ATPase Site Configuration of the RNA Helicase DbpA Probed by ENDOR Spectroscopy

Bioinformatics and Biochemical Methods to Study the Structural and Functional Elements of DEAD-box RNA Helicases

Measuring Helicase Inhibition of the DEAD-box Protein Dbp2 by Yra1

A FRET-based, Continuous Assay for the Helicase Activity of DEAD-box Proteins

A Fluorescence-Based Screening Assay for Identification of Hepatitis C Virus NS3 Helicase Inhibitors and Characterization of Their Inhibitory Mechanism

Mechanisms of HCV NS3 Helicase Monitored by Optical Tweezers

Constructing a Magnetic Tweezers to Monitor RNA Translocation at the Single-molecule Level

Probing RNA Translocases with DNA

Monitoring RNA Unwinding by the Transcription Termination Factor Rho From Mycobacterium Tuberculosis

Characterization of the Mechanisms of Transcription Termination by the Helicase Sen1

Characterization of TRAP-mediated Regulation of the B. subtilis trp Operon Using in vitro Transcription and Transcriptional Reporter Fusions in vivo

Single-molecule FRET Characterization of RNA Remodeling Induced by an Antitermination Protein

Fluorescence Reporters for Hfq Oligomerization and RNA Annealing

Fluorescence Anisotropy-based Salt-titration Approach to Characterize Protein-Nucleic Acid Interactions

Probing Hfq:RNA Interactions with Hydroxyl Radical and RNase Footprinting

Purification of Eukaryotic Exoribonucleases Following Heterologous Expression in Bacteria and Analysis of Their Biochemical Properties by in vitro Enzymatic Assays

Tips and Tricks to Probe the RNA-degrading Activities of Hyperthermophilic Archaeal β-CASP Ribonucleases.

Other Format:

Printed edition:

ISBN:

9781493922147

Access Restriction:

Restricted for use by site license.