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Autophagy in Differentiation and Tissue Maintenance : Methods and Protocols / edited by Kursad Turksen.

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Holman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
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Format:
Book
Contributor:
Turksen, Kursad, editor.
SpringerLink (Online service)
Series:
Methods in molecular biology 1064-3745 ; 1854.
Springer Protocols (Springer-12345)
Methods in Molecular Biology, 1064-3745 ; 1854
Language:
English
Subjects (All):
Cytology.
Cell Biology.
Local Subjects:
Cell Biology.
Physical Description:
1 online resource (XII, 228 pages) : 40 illustrations, 38 illustrations in color.
Contained In:
Springer eBooks
Place of Publication:
New York, NY : Springer New York : Imprint: Humana Press, 2019.
System Details:
text file PDF
Summary:
This detailed book compiles state-of-the-art protocols from researchers actively working in the area of autophagy, a crucial cellular process that regulates numerous cellular functions. In order to accelerate advances in the field, the volume explores aspects of autophagy research where a better understanding of its role is vitally important, such as in the maintenance of stem cell subpopulations and the regulation of differentiation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Autophagy in Differentiation and Tissue Maintenance: Methods and Protocols serves as an ideal guide for researchers seeking to expand our knowledge of this key cell biological action.
Contents:
Visualization and Measurement of Multiple Components of the Autophagy Flux
Mitochondrial Redox Sensor for Drosophila Female Germline Stem Cells
Induction and Detection of Autophagy in Aged Hematopoietic Stem Cells by Exposing Them to Microvesicles Secreted by HSC-Supportive Mesenchymal Stromal Cells
Skeletal Muscle Lysosomal Function via Cathepsin Activity Measurement
Autophagy in Adipocyte Differentiation
Assessing Autophagy in the Leydig Cells
Determination of Autophagy in the Caco-2 Spontaneously Differentiating Model of Intestinal Epithelial Cells
Simultaneous Detection of Autophagy and Epithelial to Mesenchymal Transition in the Non-Small Cell Lung Cancer Cells
Autophagy in Zebrafish Extraocular Muscle Regeneration
The Detection Techniques for Autophagy-Associated Cell Death Related Genes and Proteins: Gene Expression Assay and Immunohistochemistry
Cloning of Autophagy-Related MicroRNAs
Assays to Monitor Aggrephagy in Drosophila Brain
Methods for Monitoring Autophagy in Silkworm Organs
Immunofluorescence Staining Protocols for Major Autophagy Proteins Including LC3, P62, ULK1 in Mammalian Cells in Response to Normoxia and Hypoxia
Identification of Novel Autophagy Inhibitors via Cell-Based High-Content Screening
Porcine Cell-Free System to Study Mammalian Sperm Mitophagy
Monitoring and Measuring Mammalian Autophagy.
Other Format:
Printed edition:
ISBN:
9781493987481
Access Restriction:
Restricted for use by site license.

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