Antibody Engineering : Methods and Protocols / edited by Damien Nevoltris, Patrick Chames.

Format:

Book

Contributor:

Nevoltris, Damien, editor.

Chames, Patrick, editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1827.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, 1064-3745 ; 1827

Language:

English

Subjects (All):

Immunology.

Local Subjects:

Immunology.

Physical Description:

1 online resource (XVI, 543 pages) : 106 illustrations, 73 illustrations in color.

Edition:

Third edition 2018.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2018.

System Details:

text file PDF

Summary:

This detailed new edition provides complete and easy access to a variety of antibody engineering techniques. The volume explores topics such as the generation of native, synthetic, or immune antibody libraries, the selection of lead candidates via the different powerful and innovative display technologies, Fc engineering, as well as their production, characterization, and optimization of antibodies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Antibody Engineering: Methods and Protocols, Third Edition presents the reader with an extensive toolbox to create the powerful molecules of tomorrow.

Contents:

Antibody Design and Humanization via In Silico Modeling

Antibody Affinity Maturation by Computational Design

Use of IMGT® Databases and Tools for Antibody Engineering and Humanization

Construction of Human Naïve Antibody Gene Libraries

Construction of Synthetic Antibody Libraries

Construction of Histidine-Enriched Shark IgNAR Variable Domain Antibody Libraries for the Isolation of pH-Sensitive vNAR Fragments

Display Technologies for Generation of Ig Single Variable Domains

A Streamlined Approach for the Construction of Large Yeast Surface Display Fab Antibody Libraries

Phage Display and Selections on Purified Antigens

Selection of Antibodies to Transiently Expressed Membrane Proteins Using Phage Display

Selection of Antibody Fragments against Structured DNA by Phage Display

Selection of Antibody Fragments by Yeast Display

Rapid Selection of High Affinity Antibody scFv Fragments Using Ribosome Display

In Vitro Selection of Single Domain Antibody (VHH) Using cDNA Display

Sequencing and Affinity Determination of Antigen-Specific B Lymphocytes from Peripheral Blood

Expression of IgG Monoclonals with Engineered Immune Effector Functions

An IRES-Mediated Tricistronic Vector for Efficient Generation of Stable, High Level Monoclonal Antibody Producing CHO DG44 Cell Lines

Production, Purification, and Characterization of Antibody-TNF Superfamily Ligand Fusion Proteins

Chemoenzymatic Defucosylation of Therapeutic Antibodies for Enhanced Effector Functions Using Bacterial α-Fucosidases

Fc Glyco- and Fc Protein-Engineering: Design of Antibody Variants with Improved ADCC and CDC Activity

Fc Engineering: Tailored Synthetic Human IgG1-Fc Repertoire for High Affinity Interaction with FcRn at pH 6.0

Measuring Antibody-Antigen Binding Kinetics Using Surface Plasmon Resonance

Parallel Evolution of Antibody Affinity and Thermal Stability for Optimal Biotherapeutic Development

The Use of Somatic Hypermutation for the Affinity Maturation of Therapeutic Antibodies

Selection and Use of Intracellular Antibodies

Site-Specific Radioactive Labeling of Nanobodies.

Other Format:

Printed edition:

ISBN:

9781493986484

Access Restriction:

Restricted for use by site license.