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Adipose-Derived Stem Cells : Methods and Protocols / edited by Jeffrey M. Gimble, Bruce A. Bunnell.

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Holman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
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Format:
Book
Contributor:
Gimble, Jeffrey M., editor.
Bunnell, Bruce A., editor.
SpringerLink (Online service)
Series:
Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 702.
Springer Protocols (Springer-12345)
Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 702
Language:
English
Subjects (All):
Life sciences.
Cytology.
Life Sciences.
Cell Biology.
Local Subjects:
Life Sciences.
Cell Biology.
Physical Description:
1 online resource (XVI, 474 pages).
Contained In:
Springer eBooks
Place of Publication:
Totowa, NJ : Humana Press : Imprint: Humana Press, 2011.
System Details:
text file PDF
Summary:
During the past decade, a wide range of scientific disciplines have adopted the use of adipose-derived stem/stromal cells (ASCs) as an important tool for research and discovery. In Adipose-Derived Stem Cells: Methods and Protocols, experts from the field, including members of the esteemed International Federation of Adipose Therapeutics and Science (IFATS), provide defined and established protocols in order to further codify the utilization of these powerful and accessible cells. With chapters organized around approaches spanning the discovery, pre-clinical, and clinical processes, much of the emphasis is placed on human ASC, while additional techniques involving small and large animal species are included. As a volume in the highly successful Methods in Molecular Biology™ series, the detailed contributions include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Adipose-Derived Stem Cells: Methods and Protocols serves as a vital reference text for experienced researchers as well as new students on the path to further exploring the incredible potential of ASCs.
Contents:
Isolation and Culture of Rhesus Adipose-Derived Stem Cells
Isolation of Human Adipose-Derived Stem Cells from Lipoaspirates
Isolation of Murine Adipose-Derived Stem Cells
In vitro Adult Rat Adipose Tissue-Derived Stromal Cell Isolation and Differentiation
In vitro Adult Canine Adipose Tissue-Derived Stromal Cell Growth Characteristics
Adipogenic Differentiation of Adult Equine Mesenchymal Stromal Cells
Isolation and Culture of Porcine Adipose Tissue-Derived Somatic Stem Cells
Automated Isolation and Processing of Adipose-Derived Stem and Regenerative Cells
Methods for the Purification and Characterization of Human Adipose-Derived Stem Cells
Characterization of Human ASCs Using Flow Cytometry
Evaluation of Cellular and Humoral Immune Responses to Allogeneic Adipose-Derived Stem/Stromal Cells
Adipose-Derived Stromal Cells: Cytokine Expression and Immune Cell Contaminants
Gel-Based and Gel-Free Proteomic Technologies
Adipogenic Differentiation of Adipose-Derived Stem Cells
3D Culture Systems to Induce Chondrogenesis of Adipose-Derived Stem Cells
Neural Differentiation of Human ASCs
Osteogenic Differentiation Strategies for Adipose-Derived Mesenchymal Stem Cells
Generation of Adipose Stromal Cell-Derived Hepatic Cells
Adipose Stem Cell Differentiation into Smooth Muscle Cells
Endothelial and Cardiac Regeneration from Adipose Tissues
Epithelial Differentiation of Human Adipose-Derived Stem Cells
Screening for Epigenetic Target Genes that Enhance Reprogramming Using Lentiviral Delivered shRNA
Adipogenic Differentiation of Human Adipose-Derived Stem Cells on 3D Silk Scaffolds
A First Approach for the Production of Human Adipose Tissue-Derived Stromal Cells for Therapeutic Use
Viral Transduction of Adipose-Derived Stem Cells
Use of Adipose-Derived Stem Cells in High Throughput Screening to Identify Modulators of Lipogenesis
Preservation Protocols For Human Adipose Tissue-Derived Adult Stem Cells
Soft Tissue Reconstruction
Methods for Analyzing MicroRNA Expression and Function During Osteogenic Differentiation of Human Adipose Tissue-Derived Mesenchymal Stem Cells
The Generation and the Manipulation of Human Multipotent Adipose-Derived Stem Cells
Cell Sheet Technology for Tissue Engineering: The Self-Assembly Approach Using Adipose-Derived Stromal Cells
Differentiation of Adipose-Derived Stem Cells for Tendon Repair
Adipose-Derived Stem Cells for Skin Regeneration
Adipose-Derived Stem Cells for Periodontal Tissue Regeneration.
Other Format:
Printed edition:
ISBN:
9781617379604
Access Restriction:
Restricted for use by site license.

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