Neurodegeneration : Methods and Protocols / edited by Giovanni Manfredi, Hibiki Kawamata.

Format:

Book

Contributor:

Manfredi, Giovanni, editor.

Kawamata, Hibiki, editor.

SpringerLink (Online service)

Series:

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 793.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 793

Language:

English

Subjects (All):

Medicine.

Neurosciences.

Biomedicine.

Local Subjects:

Biomedicine.

Neurosciences.

Physical Description:

1 online resource (XV, 512 pages) : 100 illustrations, 9 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press : Imprint: Humana Press, 2011.

System Details:

text file PDF

Summary:

Neurodegenerative disorders, such as Alzheimer's and Parkinson's disease are a growing burden on our society. Understanding and treating these common diseases is one of the major current challenges for the biomedical field. In Neurodegeneration: Methods and Protocols, experts in the field tackle this challenge and present cutting-edge analytical and technological approaches to study the mechanisms underlying neurodegenerative processes. Organizes into five sections, chapters detail protocols for isolating and culturing cells from the nervous system, production and use of animal models, characterization of cell death, analytical tools to study disease mechanisms, and therapeutic approaches for neurodegeneration. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Neurodegeneration: Methods and Protocols serves as an ideal guide to both novice and professional researchers in the field of neurodegeneration.

Contents:

Introduction to Neurodegenerative Diseases and Related Techniques

In vivo and In vitro Determination of Cell Death Markers in Neurons

Degenerating Processes Identified by Electron Microscopic Immunocytochemical Methods

Isolation and Culture of Neurons and Astrocytes from the Mouse Brain Cortex

Isolation and Culture of Postnatal Spinal Motoneurons

Converting Human Pluripotent Stem Cells to Neural Tissue and Neurons to Model Neurodegeneration

Neural Differentiation of Induced Pluripotent Stem Cells

Developing Yeast Models of Human Neurodegenerative Disorders

Modeling Dopamine Neuron Degeneration in C. elegans

Role of the Proteasome in Fly Models of Neurodegeneration

Modeling Neurodegenerative Diseases in Zebrafish Embryos

Practical Considerations of Genetic Rodent Models for Neurodegenerative Diseases

Modeling Focal Cerebral Ischemia In vivo

Pharmacological Models of Parkison's Disease in Rodents

Behavioral Phenotyping of Mouse Models of Neurodegeneration

Characterization of Amyloid Deposits in Neurodegenerative Diseases

Detection of Protein Aggregates in Neurodegenerative Diseases

Assessment of Proteasome Impairment and Accumulation/Aggregation of Ubiquitinated Proteins in Neuronal Cultures

Measurements of Threshold of Mitochondrial Transition Pore Opening in Intact and Permeabilized Cells by Flash Photolysis of Caged Calcium

Isolation and Functional Assessment of Mitochondria from Small Amounts of Mouse Brain Tissue

Monitoring Mitophagy in Neuronal Cell Cultures

Analysis of Vesicular Trafficking in Primary Neurons by Live Imaging

Surface Trafficking of Sodium Channels in Cells and in Hippocampal Slices

Imaging Presynaptic Exocytosis in Corticostriatal Slices

Microscopic Imaging of Intracellular Calcium in Live Cells using Lifetime based Ratiometric Measurements of Oregon Green BAPTA-1

Electrophysiological Characterization of Neuromuscular Synaptic Dysfunction in Mice

Determination of Neurotransmitter Levels in Models of Parkinson's Disease by HPLC-ECD

Designing, Performing, and Interpreting a Microarray-based Gene Expression Study

Adeno-associated Viral Gene Transfer for Neurodegenerative Diseases

Specific Gene Silencing using RNAi in Cell Culture

Stem Cell Transplantation for Spinal Cord Neurodegeneration

Evaluation of Histone Deacetylase Inhibitors as Therapeutics for Neurodegenerative Diseases.

Other Format:

Printed edition:

ISBN:

9781617793288

Access Restriction:

Restricted for use by site license.