Mitochondrial Medicine : Volume I, Probing Mitochondrial Function / edited by Volkmar Weissig, Marvin Edeas.

Format:

Book

Contributor:

Weissig, Volkmar, editor.

Edeas, Marvin, 1965- editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1264.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, 1064-3745 ; 1264

Language:

English

Subjects (All):

Medicine.

Molecular biology.

Cell culture.

Biomedicine.

Molecular Medicine.

Cell Culture.

Local Subjects:

Biomedicine.

Molecular Medicine.

Cell Culture.

Physical Description:

1 online resource (XXV, 480 pages) : 108 illustrations, 66 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2015.

System Details:

text file PDF

Summary:

This expert volume covers an interdisciplinary and rapidly growing area of biomedical research comprising genetic, biochemical, pathological, and clinical studies aimed at the diagnosis and therapy of human diseases which are either caused by or associated with mitochondrial dysfunction. It dedicates itself to showcasing the tremendous efforts and the progress that has been made over the last decades in developing techniques and protocols for probing, imaging, and manipulating mitochondrial functions. Mitochondrial Medicine: Methods and Protocols, Volume I: Probing Mitochondrial Function focuses on methods being used for the assessment of mitochondrial function under physiological conditions as well as in healthy isolated mitochondria. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Mitochondrial Medicine: Methods and Protocols provides an essential source of know-how and inspiration to all researchers who are fascinated by this tiny organelle that seems so clearly to control the life and death of a single cell and whole organisms alike.

Contents:

Preparation of 'Functional' Mitochondria: A Challenging Business

Isolation and Quality Control of Functional Mitochondria

Isolation of Mitochondria-Associated ER Membranes (MAMs) and Glycosphingolipid Enriched Microdomains (GEMs) from Brain Tissues and Neuronal Cells

Fluorescence Analysis of Single Mitochondria with Nanofluidic Channels

Optical Microwell Arrays for Large Scale Studies of Single Mitochondria Metabolic Responses

Deep Resequencing of Mitochondrial DNA

Single Cell Analysis of Mitochondrial DNA

A High-Throughput Next-Generation Sequencing Assay for the Mitochondrial Genome

Rapid Mitochondrial DNA Isolation Method for Direct Sequencing

Analysis of Mitochondrial DNA and RNA Integrity by a Real-Time qPCR Based Method

Mitochondria-Targeted RNA Import

Accurate Measurement of Circulating Mitochondrial DNA Content from Human Blood Samples Using Real Time Quantitative PCR

mTRIP: An Imaging Tool to Investigate Mitochondrial DNA Dynamics in Physiology and Disease at the Single-Cell Resolution

Simultaneous Quantification of Mitochondrial ATP and ROS Production

Live-Cell Assessment of Mitochondrial Reactive Oxygen Species Using Dihydroethidine

Detection and Differentiation Between Peroxynitrite and Hydroperoxides Using Mitochondria-Targeted Arylboronic Acid

Time-Resolved Spectrometry of Mitochondrial NAD(P)H Fluorescence and Its Applications for Evaluating the Oxidative State in Living Cells

Novel Methods for Measuring the Mitochondrial Membrane Potential

High-Throughput Real-Time Analysis of Cell Oxygenation Using Intracellular Oxygen-Sensitive Probes

In Vivo Assessment of Mitochondrial Oxygen Consumption

Imaging Mitochondrial Hydrogen Peroxide in Living Cells

Simultaneous High-Resolution Measurement of Mitochondrial Respiration and Hydrogen Peroxide Production

Measurement of Mitochondrial NADH and FAD Autofluorescence in Live Cells

Mitochondrial Coenzyme Q10 Determination Via Isotope Dilution Liquid Chromatography Tandem Mass Spectrometry

Assessing the Bioenergetic Profile of Human Pluripotent Stem Cells

Integrative Methods for Studying Cardiac Energetics

Computer-Based Prediction of Mitochondria-Targeting Peptides

Prediction of Mitochondrial Protein Function by Comparative Physiology and Phylogenetic Profiling

Assessment of Posttranslational Modification of Mitochondrial Proteins

Assessment of Mitochondrial Protein Glutathionylation as Signaling for CO Pathway

High-Resolution Melting Analysis for Identifying Sequence Variations in Nuclear Genes for Assembly Factors and Structural Subunits of Cytochrome C Oxidase

Heterologous Inferential Analysis (HIA) as a Method to Understand the Role of Mitochondrial rRNA Mutations in Pathogenesis

Analysis of Mitochondrial Dysfunction During Cell Death

The Use of FLIM-FRET for the Detection of Mitochondria-Associated Protein Interactions

Assessment of Mitochondrial Ca2+ Uptake

Qualitative Characterization of the Rat Liver Mitochondrial Lipidome Using All Ion Fragmentation on an Exactive Benchtop Orbitrap MS

Characterization of Mitochondrial Populations During Stem Cell Differentiation

An Ex Vivo Model for Studying Mitochondrial Trafficking in Neurons.

Other Format:

Printed edition:

ISBN:

9781493922574

Access Restriction:

Restricted for use by site license.