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Peptide Nucleic Acids : Methods and Protocols / edited by Peter E. Nielsen.
Connect to full text Available online
View onlineHolman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
Available
- Format:
- Book
- Series:
- Methods in molecular biology 1064-3745 ; 208.
- Springer Protocols (Springer-12345)
- Methods in Molecular Biology, 1064-3745 ; 208
- Language:
- English
- Subjects (All):
- Life sciences.
- Biochemistry.
- Life Sciences.
- Biochemistry, general.
- Local Subjects:
- Life Sciences.
- Biochemistry, general.
- Physical Description:
- 1 online resource (X, 274 pages).
- Contained In:
- Springer eBooks
- Place of Publication:
- Totowa, NJ : Springer New York, 2002.
- System Details:
- text file PDF
- Summary:
- Peptide nucleic acid (PNA) technology continues to grow in importance throughout molecular biology, genetic diagnostics, and molecular medicine. In Peptide Nucleic Acids: Methods and Protocols, Peter Eigil Nielsen has assembled a critically evaluated collection of PNA protocols that are either already well established around the world, such as PCR-clamping and in situ hybridization, or that display promise of significant future impact. Basic methods for PNA oligomer synthesis and analysis have also been included. Written by experts with hands-on experience in the methods they describe, these readily reproducible protocols contain detailed step-by-step instructions, tips on avoiding pitfalls and on extending the method to other situations, and introductory material explaining the theory behind the process. Up-to-date and highly practical, Peptide Nucleic Acids: Methods and Protocols provides for both novice and experienced researchers a thorough and easy introduction to the optimal use of today's PNA technology across a wide variety of fields, including molecular biology, medicinal chemistry, genetic diagnostics, and drug development.
- Contents:
- PNA Technology
- Chemistry
- Solid Phase Synthesis of PNA Oligomers
- Synthesis of PNA-Peptide Conjugates
- Parallel Synthesis of PNA-Peptide Conjugate Libraries
- Thermodynamics of PNA Interactions with DNA and RNA
- PNA Technology
- Measurement of PNA Binding to Double-Stranded DNA
- PNA-Mediated Immobilization of Supercoiled DNA
- PNA Openers and Their Applications
- Colorimetric Detection of PNA-DNA Hybridization Using Cyanine Dyes
- Diagnostics
- PNA-Mediated PCR Clamping
- PNA Clamping Techniques for the Determination of Oncogene Mutations
- PNA Fluorescent In Situ Hybridization for Rapid Microbiology and Cytogenetic Analysis
- Detection of Point Mutations Using PNA-Containing Electrophoresis Matrices
- Antisense and Antigene
- Lipid-Mediated Introduction of Peptide Nucleic Acids Into Cells
- Synthesis of Cell-Penetrating Peptide-PNA Constructs
- Antisense Inhibition of Bacterial Gene Expression and Cell Growth
- In Vitro Transcription from Peptide Nucleic Acid/DNA Strand Displacement Loops
- Animal Studies
- In Vitro and In Vivo Studies on the Pharmacokinetics and Metabolism of PNA Constructs in Rodents.
- Other Format:
- Printed edition:
- ISBN:
- 9781592592906
- Access Restriction:
- Restricted for use by site license.
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