Amyloid Proteins : Methods and Protocols / edited by Einar M. Sigurdsson.

Format:

Book

Contributor:

Sigurdsson, Einar M., editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 299.

Springer Protocols (Springer-12345)

Methods in Molecular Biology™, 1064-3745 ; 299

Language:

English

Subjects (All):

Life sciences.

Biochemistry.

Life Sciences.

Biochemistry, general.

Local Subjects:

Life Sciences.

Biochemistry, general.

Physical Description:

1 online resource (408 pages) : 156 illustrations, 1 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2005.

System Details:

text file PDF

Summary:

In recent years, various in vitro and in vivo techniques, transgenic technologies, and novel animal models have been developed to understand amyloid diseases-such as Alzheimer's disease and some forms of diabetes-and to screen for drugs that can block amyloid formation and/or disassemble its associated fibrils. In Amyloid Proteins: Methods and Protocols, internationally known researchers describe in step-by-step detail their best techniques for studying these types of proteins and their involvement in the etiology, pathogenesis, diagnosis, and therapy of amyloid diseases. The contributors provide proven methods for the preparation of amyloid and its precursors (oligomers and protofibrils), in vitro assays and analytical techniques for their study, and cell culture models and assays for the production of amyloid proteins. Additional chapters present readily reproducible techniques for amyloid extraction from tissue, its detection in vitro and in vivo, as well as nontransgenic methods for developing amyloid mouse models. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and highly practical, Amyloid Proteins: Methods and Protocols offers both skilled and novice investigators a proven collection of standard and novel techniques for studying amyloid proteins and peptides, as well as their relation to amyloid diseases.

Contents:

In Vitro Assays

Preparation of Aggregate-Free,Low Molecular Weight Amyloid? for Assembly and Toxicity Assays

Determination of Peptide Oligomerization State Using Rapid Photochemical Crosslinking

In Vitro Preparation of Prefibrillar Intermediates of Amyloid-? and ? Synuclein

Purification of Recombinant Tau Protein and Preparation of Alzheimer-Paired Helical Filaments In Vitro

Cyclic Amplification of Protein Misfolding and Aggregation

X-Ray Diffraction Studies of Amyloid Structure

Molecular Electron Microscopy Approaches to Elucidating the Mechanisms of Protein Fibrillogenesis

Time -Lapse Atomic Force Microscopy in the Characterization of Amyloid-Like Fibril Assembly and Oligomeric Intermediates

Fourier Transform Infrared and Circular Dichroism Spectroscopies for Amyloid Studies

Quasielastic Light Scattering for Protein Assembly Studies

Intrinsic Fluorescent Detection of Tau Conformation and Aggregation

Quantitative Measurement of Fibrillogenesis by Mass Spectrometry

Cell Culture Assays

Isolation and Culturing of Human Vascular Smooth Muscle Cells

Murine Cerebrovascular Cells as a Cell Culture Model for Cerebral Amyloid Angiopathy

Purification of Human Wild-Type or Variant Cystatin C From Conditioned Media of Transfected Cells

Prion Propagation in Cell Culture

In Vivo-Related Assays

Preparation and Propagation of Amyloid-Enhancing Factor

Purification of Amyloid Protein AA Subspecies From Amyloid-Rich Human Tissues

Purification of Transthyretin and Transthyretin Fragments From Amyloid-Rich Human Tissues

Extraction and Chemical Characterization of Tissue-Deposited Proteins From Minute Diagnostic Biopsy Specimens

Tissue Processing Prior to Protein Analysis and Amyloid-ß Quantitation

ELISA Method for Measurement of Amyloid-ß Levels

Histological Staining of Amyloid-ß in Mouse Brains

The Mouse Model for Scrapie

Radiolabeling of Amyloid-ß Peptides

In Vivo Imaging of Amyloid-ß Deposits in Mouse Brain With Multiphoton Microscopy

Magnetic Resonance Imaging of Amyloid Plaques in Transgenic Mice.

Other Format:

Printed edition:

ISBN:

9781592598748

Access Restriction:

Restricted for use by site license.