Protein Downstream Processing : Design, Development and Application of High and Low-Resolution Methods / edited by Nikolaos E. Labrou.

Format:

Book

Contributor:

Labrou, Nikolaos E., editor.

SpringerLink (Online service)

Series:

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 1129.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 1129

Language:

English

Subjects (All):

Life sciences.

Proteins.

Life Sciences.

Protein Science.

Local Subjects:

Life Sciences.

Protein Science.

Physical Description:

1 online resource (XV, 555 pages) : 121 illustrations, 62 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press : Imprint: Humana Press, 2014.

System Details:

text file PDF

Summary:

Proteins are the most diverse group of biologically important substances. With the recent technological advances in the genomics area and the efforts in proteomics research, the rate of discovery for new proteins with unknown structure and function has increased. These proteins generated from genomic approaches present enormous opportunities for research and industrial application. Protein Downstream Processing: Design, Development and Application of High and Low-Resolution Methods is a compilation of chapters within the exciting area of protein purification designed to give the laboratory worker the information needed to design and implement a successful purification strategy. It presents reliable and robust protocols in a concise form, emphasizing the critical aspects on practical problems and questions encountered at the lab bench. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Protein Downstream Processing: Design, Development and Application of High and Low-Resolution Methods will be an ideal source of scientific information to advanced students, junior researchers, and scientists involved in health sciences, cellular and molecular biology, biochemistry, and biotechnology and other related areas in both academia and industry.

Contents:

Protein Purification: An Overview

Establishment of a Design Space for Biopharmaceutical Purification Processes Using DoE

High Throughput Process Development: I. Process Chromatography

High Throughput Process Development: II. Membrane Chromatography

Media Selection in Ion Exchange Chromatography in a Single Microplate

High Throughput Screening of Dye-Ligands for Chromatography

Measurement of Uptake Curves and Adsorption Isotherms by Automated Microscale Chromatography Pipette Tips

Recovery of Recombinant Proteins from Plants Using Aqueous Two-Phase Partitioning Systems: An Outline

Aqueous Two-Phase Systems Strategies for the Recovery of Proteins from Plants

Aqueous Two Phase Assisted Precipitation of Proteins: A Platform for Isolation of Process Related Impurities from Therapeutic Proteins

Simultaneous Purification and Refolding of Proteins by Affinity Precipitation and Macro-(Affinity Ligand) Facilitated Three Phase Partitioning (MLFTPP)

Co-Expression and Co-Purification of Antigen-Antibody Complexes in Bacterial Cytoplasm and Periplasm

Immunoglobulin Purification by Caprylic Acid

Affinity Tags in Protein Purification and Peptide Enrichment: An Overview

Expression and Purification of Large Active GST Fusion Enzymes

Synthetic Ligand Affinity Chromatography. Purification of Human Serum Albumin and Related Fusion Proteins

Zbasic - A Purification Tag for Selective Ion-Exchange Recovery

An Orthogonal Fusion Tag for Efficient Protein Purification

Phage Display of Engineered Binding Proteins

Biomimetic Affinity Ligands for Protein Purification

Synthesis and Application of Dye-Ligand Affinity Adsorbents

Peptide Affinity Chromatography Based on Combinatorial Strategies for Protein Purification

Affinity Chromatography of Proteins on Monolithic Columns

Sample Displacement Batch Chromatography of Proteins

Analysis of Host-Cell Proteins in Biotherapeutic Proteins by LC/MS Approaches

Preparation of Monolithic Affinity Media for Nano-Liquid Chromatography Applications

Proteomic Analysis of Complex Protein Samples by MALDI TOF Mass Spectrometry

Modern Bioanalysis of Proteins by Electrophoretic Techniques

Protein Structure Validation and Analysis with X-Ray Crystallography

Measuring Binding Constants of His-Tagged Proteins using Affinity Chromatography and Ni-NTA Immobilized Enzymes

Stabilization of Therapeutic Proteins in Aqueous Solutions and Freeze-Dried Solids: An Overview

Stabilization of Protein by Freeze-Drying in the Presence of Trehalose: A Case Study of Tubulin

G Protein-Coupled Receptor Expression and Purification.- (Hyper)thermophilic Enzymes: Production and Purification

Affinity Chromatography for Antibody Purification

Screening and Purification of Recombinant Lignocellulolytic Enzymes

Purification of PEGylated Proteins, with the Example of PEGylated lysozyme and PEGylated scFv

One-Step Purification of Glutamate decarboxylase from E. coli Using Aqueous Two Phase System.

Other Format:

Printed edition:

ISBN:

9781627039772

Access Restriction:

Restricted for use by site license.