Parkinson's Disease : Methods and Protocols / edited by M. Maral Mouradian.

Format:

Book

Contributor:

Maral Mouradian, M., editor.

SpringerLink (Online service)

Series:

Methods in molecular medicine 1543-1894 ; 62.

Springer Protocols (Springer-12345)

Methods in Molecular Medicine™, 1543-1894 ; 62

Language:

English

Subjects (All):

Medicine.

Neurology.

Medicine & Public Health.

Local Subjects:

Medicine & Public Health.

Neurology.

Physical Description:

1 online resource (XI, 317 pages).

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2001.

System Details:

text file PDF

Summary:

The use of molecular technologies to study Parkinson's disease in the laboratory is beginning to have a major impact in clinical medicine. In Parkinson's Disease: Methods and Protocols, internationally recognized biomedical investigators describe in detail the major techniques employed in molecular and cellular studies of Parkinson's disease and basal ganglia function, adding along the way considerable background information about the rationale and utility of these methods, as well as about fundamental issues in Parkinson's disease. Widely varied methods are covered, including genetic analyses, molecular pathogenetic investigations of dopaminergic neuronal degeneration, biochemical studies of nigrostriatal neural circuitry, and molecular therapies, such as gene therapy and neural stem cells. Comprehensive and timely, Parkinson's Disease: Methods and Protocols offers scientists and clinicians interested in Parkinson's and related neurodegenerative disorders the major cutting-edge methods-in a readily reproducible format-needed to effectively exploit the potential of cell and molecular biology for elucidating disease mechanisms and for speeding the emergence of novel and more effective therapeutics.

Contents:

Genetics

Point Mutations in the ?-Synuclein Gene

Autosomal Recessive Juvenile Parkinsonism (AR-JP): Genetic Diagnosis

Molecular Pathogenetic Studies

Parkinson's Disease, Dementia with Lewy Bodies, and Multiple System Atrophy as ?-Synucleinopathies

?-Synuclein/Amyloid Interactions

Functional Defect Conferred by the Parkinson's Disease-Causing ?-Synuclein (Ala30Pro) Mutation

Molecular Biology of Dopamine-Induced Apoptosis

Animal Models of Induced Apoptotic Death in the Substantia Nigra

Apoptotic Morphology in Phenotypically Defined Dopaminergic Neurons of the Substantia Nigra

The Role of Nitric Oxide in Parkinson's Disease

Oxidative Stress Indices in Parkinson's Disease

Molecular Aspects of Basal Ganglia Function

Quantification of Tyrosine Hydroxylase mRNA

Immunochemical Analysis of Dopamine Transporters in Parkinson's Disease

Dopamine Transporter and Vesicular Monoamine Transporter Knockout Mice

Transcription Mechanisms for Dopamine Receptor Genes

Detection of DNA/Protein Interactions in Dopamine Receptor Genes

In Situ Hybridization on Brain Tissue

Regulation of Striatal N-Methyl-D-Aspartate Receptor (NMDAR) Function by Phosphorylation of its Subunits in Parkinsonian Rats

Molecular Therapies

Chronic Intracerebral Delivery of Trophic Factors via a Programmable Pump as a Treatment for Parkinsonism

Grafting Genetically Engineered Cells into the Striatum of Nonhuman Primates

Encapsulated Cell Implants as a Novel Treatment for Parkinson's Disease

Neural Stem Cell Technology as a Novel Treatment for Parkinson's Disease.

Other Format:

Printed edition:

ISBN:

9781592591428

Access Restriction:

Restricted for use by site license.