Interferon Methods and Protocols / edited by Daniel J. J. Carr.

Format:

Book

Contributor:

Carr, Daniel J. J., editor.

SpringerLink (Online service)

Series:

Methods in molecular medicine 1543-1894 ; 116.

Springer Protocols (Springer-12345)

Methods in Molecular Medicine™, 1543-1894 ; 116

Language:

English

Subjects (All):

Medicine.

Immunology.

Biomedicine.

Local Subjects:

Biomedicine.

Immunology.

Physical Description:

1 online resource (240 pages) : 51 illustrations, 1 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2005.

System Details:

text file PDF

Summary:

Although some interferons (IFNs) are widely used for their antiviral, antiproliferative, and immunoregulatory properties, there are many IFN-stimulatory genes that have unknown functions, but hold great promise in medicine and biomedical research. In Interferon Methods and Protocols, leading experts actively engaged in interferon research describe in detail their optimized methods to measure type I interferon efficacy as an antiproliferative or an antiviral agent. These cutting-edge techniques range from the simple to the highly complex and serve to illuminate the signaling cascades and the activation of enzymatic pathways prompted by interferon. Two chapters add an historical perspective to the field in general, discussing the successes and failures of past and present day technology, as well as the appropriate directions for consideration of future investigations. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Interferon Methods and Protocols offers researchers powerful tools not only to ascertain the functions of IFN-stimulatory gene products, but also to identify additional molecular pathways that will clarify our understanding of the many biological events influenced by IFNs.

Contents:

Biological and Clinical Basis for Molecular Studies of Interferons

Interferon Research

Virus Infection and the Interferon Response

Genomic DNA Affinity Chromatography

Protein Engineering of Interferon Alphas

Assays for the Interferon-Induced Enzyme 2?,5? Oligoadenylate Synthetases

A Convenient and Sensitive Fluorescence Resonance Energy Transfer Assay for RNase L and 2?,5? Oligoadenylates

Regulation of Murine Interferon Regulatory Factor Gene Expression in the Central Nervous System Determined by Multiprobe RNase Protection Assay

Mitogen-Activated Protein Kinase Pathways in Interferon Signaling

Development of an Interferon-Based Cancer Vaccine Protocol

Type I Interferons as Regulators of the Differentiation/Activation of Human Dendritic Cells

Flow Cytometric Techniques for Studying Plasmacytoid Dendritic Cells in Mixed Populations

Analysis of Anti-Interferon Properties of the Herpes Simplex Virus Type I ICP0 Protein

Interferon Subtype Gene Therapy for Regulating Cytomegalovirus Disease

Transfection of Müller Cells With Type I Interferon Transgenes.

Other Format:

Printed edition:

ISBN:

9781592599394

Access Restriction:

Restricted for use by site license.